Arabidopsis nitric oxide synthases (AtNOS1) is a member of the circularly permuted guanosine triphosphatase (cGTPase) family and has guanine-binding motifs (G motifs) in the central domain region. It displays no sequence similarity to any known mammalian NOS isoforms.
synthetic peptide corresponding to amino acids 544-561 located at the C-terminus of AtNOS1.
Application
Anti-AtNOS1 (C-terminal) antibody produced in rabbit may be used in immunoblotting.
Biochem/physiol Actions
Arabidopsis nitric oxide synthases (AtNOS1) is needed for nitric oxide (NO) synthesis in vivo. NOS uses arginine and nicotinamide adenine dinucleotide phosphate (NADPH) and is activated by calmodulin and calcium (Ca2+). AtNOS1 plays a key role in organ growth, and abscisic acid (ABA)-induced stomatal movements. In Arabidopsis, NOS is targeted to the mitochondria of leaves, roots and appears to protect against oxidative damage and dark-induced senescence.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
The Arabidopsis thaliana protein nitric oxide synthase1 (NOS1) is needed for nitric oxide (NO) synthesis and signaling during defense responses, hormonal signaling, and flowering. The cellular localization of NOS1 was examined because it is predicted to be a mitochondrial protein.
The Journal of biological chemistry, 283(47), 32957-32967 (2008-09-20)
AtNOS1 was previously identified as a potential nitric-oxide synthase (NOS) in Arabidopsis thaliana, despite lack of sequence similarity to animal NOSs. Although the dwarf and yellowish leaf phenotype of Atnos1 knock-out mutant plants can be rescued by treatment with exogenous
Questions
Reviews
★★★★★ No rating value
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
