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N9411

SAFC

Nerve growth factor receptor/Fc chimera from mouse

recombinant, expressed in mouse NSO cells, lyophilized powder, suitable for cell culture

Synonym(s):

NGF R, p75 Neurotrophin Receptor

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About This Item

MDL number:
MFCD03703185
UNSPSC Code:
12352200

recombinant

expressed in mouse NSO cells

assay

≥90% (SDS-PAGE)

form

lyophilized powder

mol wt

apparent mol wt 75-100 kDa by SDS-PAGE (reducing)
calculated mol wt 50.4 kDa

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

UniProt accession no.

Q9Z0W1

storage temp.

−20°C

Gene Information

mouse ... Ngfr(18053)

Biochem/physiol Actions

The NGF receptor also named low-affinity NGF receptor (LNGFR), a potent NGF antagonist, is a 75 kDa, type I transmembrane protein consisting of an extracellular domain, which contains four cysteine-rich domains responsible for ligand binding. The cytoplasmic region of the receptor contains a subtype 2 death domain. Nerve Growth Factor Receptor (NGF R), binds NGF and other neurotrophins including BDNF, NT-3, and NT-4 with similar affinity. NGF-R is necessary for regulating neuronal growth, cell migration, gene expression, differentiation, and cell death during development of the central and peripheral nervous system.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline.

Preparation Note

Nerve growth factor receptor/Fc chimera mouse is a chimeric protein expressed in a mouse myeloma cell line, NSO. A DNA sequence encoding the signal peptide from human CD33 joined with the extracellular domain of mouse nerve growth factor receptor (NGF R)1 is fused to the Fc region of human IgG1 via a peptide linker. Mature recombinant mouse NGF R /Fc is a disulfide linked homodimeric protein. Based on N-terminal amino acid sequencing, the mature protein starts at Gly 17. The recombinant mouse NGF R/Fc monomer has a calculated molecular mass 50.4 kDa. As a result of glycosylation, recombinant mouse NGF receptor/Fc migrates with an apparent molecular mass of 75-100 kDa in SDS-PAGE.

Analysis Note

The biological activity is measured by its ability to inhibit recombinant mouse β-NGF-induced proliferation of TF-1 cells.

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
050M1177
109K1088
084K0895

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Wea-Lung Lin et al.
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The previous data showed that caffeic acid phenethyl ester (CAPE), a component of propolis, possesses inducing cell cycle arrest and antiproliferation effect on C6 glioma cells in vitro and in vivo. In the present study, C6 glioma cells treated with
C Tuffereau et al.
The EMBO journal, 17(24), 7250-7259 (1998-12-19)
A random-primed cDNA expression library constructed from the mRNA of neuroblastoma cells (NG108) was used to clone a specific rabies virus (RV) receptor. A soluble form of the RV glycoprotein (Gs) was utilized as a ligand to detect positive cells.
S X Bamji et al.
The Journal of cell biology, 140(4), 911-923 (1998-03-21)
To determine whether the p75 neurotrophin receptor (p75NTR) plays a role in naturally occurring neuronal death, we examined neonatal sympathetic neurons that express both the TrkA tyrosine kinase receptor and p75NTR. When sympathetic neuron survival is maintained with low quantities

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