N9287

Monoclonal Anti-NBS1 (Nibrin) antibody produced in mouse

~2 mg/mL, clone NBS1-501, purified immunoglobulin, buffered aqueous solution

• Synonym(s): Anti-Nijmegen Breakage Syndrome, Anti-p95 Protein of NBS1.MRE11/RAD50 Complex
• MDL number: MFCD03455620
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: NBS1-501, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~95 kDa
• species reactivity: human
• concentration: ~2 mg/mL
• technique(s): immunocytochemistry: suitable; immunoprecipitation (IP): suitable; microarray: suitable; western blot: 2-4 μg/mL using nuclear extract of HEK 293T expressing recombinant human NBS1
• isotype: IgG1
• UniProt accession no.: O60934
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... NBN(4683)

Description

General description

Monoclonal Anti-NBS1 (Nibrin) (mouse IgG1isotype) is derived from the hybridoma NBS1-501 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a synthetic peptide of human NBS1. Nibrin is mapped to human chromosome 8q21.3. The encoded protein has two domains found in the cell cycle checkpoint proteins, forkhead-associated domain (FHA), and an adjacent breast cancer carboxy-terminal domain (BRCT).

Immunogen

synthetic peptide corresponding to amino acids 206-220 of human NBS1.

Application

Monoclonal Anti-NBS1 (Nibrin) antibody produced in mouse has been used in:

• antibody microarray
• immunocytochemistry
• immunoblotting
• immunoprecipitation
• enzyme linked immunosorbent assay (ELISA)

Biochem/physiol Actions

NBS1(Nibrin) was first isolated as a protein involved in DNA repair through analysis of mutations in patients with this syndrome. p95/NBS1(Nibrin) deficiency abrogates the formation of the Meiotic recombination 11 homolog 1(MRE11) / DNA repair protein RAD50 ionizing radiation-induced foci, revealing a molecular link between double-stranded break repair (DSB) and cell cycle checkpoint functions. The phenotypic similarities between ataxia-telangiectasia (AT) and Nijmegen breakage syndrome had suggested that ataxia-telangiectasia mutated gene (ATM) and NBS1 functions in a common signaling pathway. This was confirmed by the finding that in response to ionizing radiation, NBS1is phosphorylated in Ser343 in an ataxia-telangiectasia mutated ATM-dependent manner.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable