N8414
Anti-NUMB
~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym(s):
Anti-S171
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~70 kDa
species reactivity
rat, human
concentration
~1.5 mg/mL
technique(s)
western blot: 1-2 μg/mL using rat brain extract (S1 fraction) and A431 cell lysate
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... NUMB(8650)
mouse ... Numb(18222)
rat ... Numb(29419)
General description
NUMB endocytic adaptor protein (Numb) is a conserved adapter protein. NUMB gene is mapped to human chromosome 14. The mammalian Numb gene gives rise to at least four alternatively spliced transcripts that produce four protein isoforms, Numb 1-4. Numb 1 and 3 have a proline-rich region.
Specificity
Anti-NUMB specifically recognizes human and rat NUMB.
Application
Anti-NUMB antibody may be used in immunoblotting.
Biochem/physiol Actions
Numb is an antagonist of the notch. It plays a role in the neural differentiation in the central nervous system (CNS) and self-renewal of neural stem cells (NSCs). Loss of Numb function in mice results in embryonic lethality and precocious neuronal differentiation. It has been suggested that a switch in Numb isoform expression is a critical step in cortical development. The proliferation of neural crest stem cells (NCSCs) is promoted by the proline-rich region (PRRL) of the isoform 1 and 3. The ectopic expression of Numb2 or Numb4 isoforms devoid of the proline?rich region mediates enhanced neuronal differentiation. The loss of Numb may impact the epithelial-to-mesenchymal transition (EMT).
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Product Selector Tool.
Choose from one of the most recent versions:
Certificates of Analysis (COA)
Lot/Batch Number
Don't see the Right Version?
If you require a particular version, you can look up a specific certificate by the Lot or Batch number.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Elisa Tassano et al.
Molecular cytogenetics, 7, 49-49 (2014-08-01)
Interstitial deletions of chromosome bands 14q24.1q24.3 are very rare with only three reported cases. We describe a 7-year-old boy with a 5.345 Mb de novo interstitial deletion at 14q24.1q24.3 band detected by array-CGH who had a complex phenotype characterized by seizures
Mahmud Bani-Yaghoub et al.
Developmental dynamics : an official publication of the American Association of Anatomists, 236(3), 696-705 (2007-01-27)
Loss of numb function suggests that numb maintains progenitors in an undifferentiated state. Herein, we demonstrate that numb1 and numb3 are expressed in undifferentiated cortical progenitors, whereas numb2 and numb4 become prominent throughout differentiation. To further assess the role of
Martina Zobel et al.
The Journal of cell biology, 217(9), 3161-3182 (2018-08-01)
The endocytic protein NUMB has been implicated in the control of various polarized cellular processes, including the acquisition of mesenchymal migratory traits through molecular mechanisms that have only been partially defined. Here, we report that NUMB is a negative regulator
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service
