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N7155

Sigma-Aldrich

Anti-Nitric Oxide Synthase, Brain (251-270) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):

Anti-bNOS

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About This Item

MDL number:
MFCD01092427
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 150-160 kDa

species reactivity

human, rat

technique(s)

microarray: suitable
western blot: 1:10,000 using rat brain extract

UniProt accession no.

P29475

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... NOS1(4842)
rat ... Nos1(24598)

General description

Nitric Oxide Synthase, Brain (NOS1) is mapped to human chromosome 12q24.22. The NOS isoform found in neurons is a 150 kDa protein. It is also termed as brain NOS (bNOS). Human bNOS and endothelial NOS (eNOS) share 52% amino acid identity, and rat and human bNOS share 93% amino acid identity.

Immunogen

synthetic peptide corresponding to nitric oxide synthase (NOS) of rat brain origin (bNOS, amino acids 251-270) conjugated to KLH. The immunogen sequence is highly conserved in human bNOS.

Application

Anti-Nitric Oxide Synthase, Brain (251-270) antibody produced in rabbit has been used in:
  • double immunolabelling,
  • immunofluorescence
  • western blotting

Biochem/physiol Actions

Nitric Oxide Synthase, Brain (bNOS) is present also in skeletal muscle, where it is complexed with dystrophin and is absent in Duchenne muscular dystrophy (DMD). Neuronal and endothelial NOS are constitutively expressed and are dependent on Ca2+ calmodulin for nitric oxide (NO) production.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Therapeutic strategies to address neuronal nitric oxide synthase deficiency and the loss of nitric oxide bioavailability in Duchenne Muscular Dystrophy
Timpani CA, et al.
Orphanet Journal of Rare Diseases, 12(1), 100-100 (2017)
Endothelial and neuronal nitric oxide synthases are present in the suprachiasmatic nuclei of Syrian hamsters and rats
Caillol M, et al.
The European Journal of Neuroscience, 12(2), 649-661 (2000)
A melon pulp concentrate rich in superoxide dismutase reduces stress proteins along the gastrointestinal tract of pigs
Lalles JP, et al.
Nutrition, 27(3), 358-363 (2011)
nNOS-expressing neurons in the ventral tegmental area and substantia nigra pars compacta
Paul EJ, et al.
eNeuro, 5(5), 100-100 (2018)
Expression of nitric oxide synthase in the retina of monocular deprivation amblyopia rats
Wang QM, et al.
European Review for Medical and Pharmacological Sciences, 22(7), 1879-1883 (2018)
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