western blot: 0.5-1 μg/mL using whole cell extract of E. coli expressing NPTII western blot: 1-2 μg/mL using extract (cytosolic fraction from the leaves of transgenic Nicotiana tobacum expressing E. coli NPTII
shipped in
dry ice
storage temp.
−20°C
Specificity
The antibody recognizes bacterial NPTII expressed in transgenic tobacco plants and E. coli.
Immunogen
synthetic peptide corresponding to amino acids 250-264 located at the C-terminus of E. coli NPTII, conjugated to KLH.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
The lateral organization of lipid components within membranes is usually investigated with fluorescence microscopy, which, though highly sensitive, introduces bulky fluorophores that might alter the behavior of the components they label. Secondary ion mass spectroscopy performed with a NanoSIMS 50
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