N5665
BLOCKED P-NITROPHENYL-ALPHA-D-MALTOHEPTAOSIDE
powder
Synonym(s):
BpNPG7
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.28
Recommended Products
product name
BLOCKED P-NITROPHENYL-ALPHA-D-MALTOHEPTAOSIDE,
form
powder
Quality Level
mol wt
1362
impurities
≤3% Water content (Karl Fischer)
color
white to yellow
shipped in
dry ice
storage temp.
−20°C
General description
This product is useful as a substrate for determination of α-amylase activity. This oligosaccharide contains a blocking group at the non-reducing end, which prevents exoglucosidase from attacking the substrate molecule. Amylase can still cleave the substrate internally, which releases short p-nitrophenol-linked (pNP-linked) oligosaccharides. After removal of the blocking group, auxiliary enzymes such as glucoamylase and maltase can release the chromogen. Glucoamylase accelerates this reaction via action on the longer chains. Maltase then separates these longer chains from the pNP moiety.
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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DNA typing after α-amylase test.
Rapone, C. et al.
International Congress Series, 1239, 911-915 (2003)
Bruna Yuki Tagomori et al.
3 Biotech, 11(1), 13-13 (2021-01-15)
This work reports the amy1 gene cloning from Massilia timonae CTI-57, and its successful expression in Escherichia coli Rosetta™ (DE3) from the pTRCHis2B plasmid. The recombinant AMY1 protein had 47 kDa, and its modeled structure showed a monomer composed of three
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