N5538

Monoclonal Anti-3-Nitrotyrosine antibody produced in mouse

clone 18G4, purified immunoglobulin, buffered aqueous glycerol solution

• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 18G4, monoclonal
• form: buffered aqueous glycerol solution
• mol wt: antigen ~55 kDa
• species reactivity: wide range
• technique(s): western blot: 1:1,000 using mouse and rat brain
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: nitration (Tyr)
• Gene Information: human ... NOS1(4842)

Description

General description

3-nitrotyrosine (3-NT) is synthesized by the incorporation of nitro triatomic group (-NO₂) at the third position of the phenolic ring in the tyrosine residue, in response to the production of peroxynitrite in the oxidative and nitrosative stress.

Immunogen

3-Nitrotyrosine-KLH.

Application

Monoclonal Anti-3-Nitrotyrosine antibody produced in mouse has been used in western blot analysis and immunohistochemistry.

Biochem/physiol Actions

3-Nitrotyrosine (3-NT) in tissue extracts is a marker for the determination of protein damage induced by peroxynitrite. 3-NT is used as a biomarker to determine the protein nitration, associated with various disorders. It is implicated in diseases characterized by endothelial dysfunction as in stroke, myocardial infarction, atherosclerosis and diabetes.

Target description

Monoclonal Anti-3-Nitrotyrosine recognizes 3-nitrotyrosine in most species. Protein tyrosine nitration results in a post-translational modification that is increasingly receiving attention as an important component of nitric oxide signaling.

Physical form

Solution in 20 mM sodium phosphate, 150 mM sodium chloride, 50% glycerol, and 3 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable