N5287

Monoclonal Anti-Nuf2 antibody produced in mouse

~2 mg/mL, clone 28-37, purified immunoglobulin, buffered aqueous solution

• MDL number: MFCD07785409
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 28-37, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~52 kDa
• species reactivity: human
• concentration: ~2 mg/mL
• technique(s): immunocytochemistry: suitable; immunoprecipitation (IP): suitable; indirect ELISA: suitable; microarray: suitable; western blot: 0.5-1 μg/mL using total cell extract of A549 cells
• isotype: IgG1
• UniProt accession no.: Q9BZD4
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... NUF2(83540)

Description

General description

Monoclonal Anti-Nuf2 (mouse IgG1isotype) is derived from the hybridoma 28-37 produced by the fusion of mouse myeloma cells (PAI cells) and splenocytes from BALB/c mice. NUF2 gene is mapped to human chromosome 1q23.3. NUF2 protein is a component of the kinetochore complex.

NDC80 kinetochore complex component, NUF2 is also called as cell division associated 1 (CDCA1).

Specificity

Monoclonal Anti-Nuf2 recognizes human Nuf2.

Immunogen

recombinant full length human Nuf2.

Application

Monoclonal Anti-Nuf2 antibody produced in mouse may be used in:

• enzyme-linked immunosorbent assay (ELISA)
• immunoblotting
• immunocytochemistry
• immunoprecipitation

Biochem/physiol Actions

Downregulation of the NUF2 protein by RNA interference results in the failure of the kinetochores to form attachments to the spindle microtubules. As a consequence, cells are blocked in the prometaphase stage with an active spindle checkpoint and undergo cell death.

NDC80 kinetochore complex component, NUF2 is highly expressed in glioma. It has important roles in attachment of the microtubule to the kinetochore and in chromosome segregation. NUF2 is also involved in enhancing cell growth in hepatocellular carcinoma.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in “frost-free” freezers is also not recom-mended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.