Skip to Content
MilliporeSigma
All Photos(1)

Key Documents

N5040

Sigma-Aldrich

Endothelial Nitric Oxide Synthase of Human

recombinant, expressed in E. coli

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12352200

recombinant

expressed in E. coli

form

solution

specific activity

70-100 units/mg protein

shelf life

6‑12 mo.

shipped in

dry ice

storage temp.

−70°C

Components

40mM EPPS buffer pH 7.5
100mM Nacl
5% Glycerol
5μM H4B
100μM L-arginine

Unit Definition

One unit of enzyme activity represents the amount of enzyme that produce 1nmole of nitric oxide per minute at 37°C in 40mM Tris-HCl, or Hepes or EPPs buffer, pH 7.5 containg 5-10μM Oxyhemoglobin. 1mM NADPH, 2μM FMN/FAD, 5μM H4B, 100μM L-arginine, 50units/ml Catalase, 5units/ml SOD, 0.1mg/ml BSA, 35μl of 9mM EDTA and 35μl of 150mg/ml CaM and 12.5mM CaCl2 in a final volume 1ml. The reaction volume can be adjusted to 250μl according to experimental condition.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Questions

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service