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N3662

Sigma-Aldrich

Monoclonal Anti-Neurofibromin antibody produced in mouse

clone NFn27b, ascites fluid

Synonym(s):

Anti-Merlin Schwannomin, Anti-SCH

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About This Item

MDL number:
MFCD03845618
UNSPSC Code:
12352203

biological source

mouse

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

NFn27b, monoclonal

species reactivity

rat, mouse, human

technique(s)

immunohistochemistry: suitable
microarray: suitable
western blot: 1:100-1:200 using rat brain cytosolic extract

UniProt accession no.

P21359

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... NF1(4763)
mouse ... Nf1(18015)
rat ... Nf1(24592)

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General description

Neurofibromin 1 (NF1) is tumor suppressor. The gene encoding it is localized on human chromosome 17q11.2.

Immunogen

synthetic peptide corresponding to amino acids 27-41 located in the N-terminus of human neurofibromin.

Biochem/physiol Actions

Neurofibromin 1 (NF1) acts as a negative regulator of RAS (a GTPase). Mutations in the gene encoding it have been associated with neurofibromatosis type 1 and melanoma.

Suitability

Clone NFn27b is recommended over clone NFn27a (Product Code N 3537) for immunohistochemistry assays.

Physical form

Ascites fluid with 15 mM sodium azide as a preservative.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificates of Analysis (COA)

Lot/Batch Number
105K4761

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M Adelita Vizcaíno et al.
Human pathology, 46(9), 1323-1330 (2015-07-21)
Recent studies have identified somatic alterations in the gene encoding for neurofibromin (NF1) in a subset of glioblastoma (GBM), usually associated with the mesenchymal molecular subtype. To understand the significance of NF1 genetic alterations in diffuse gliomas in general, we
Meng-Chang Hsiao et al.
American journal of human genetics, 97(2), 238-249 (2015-07-21)
Genomic rearrangements can cause both Mendelian and complex disorders. Currently, several major mechanisms causing genomic rearrangements, such as non-allelic homologous recombination (NAHR), non-homologous end joining (NHEJ), fork stalling and template switching (FoSTeS), and microhomology-mediated break-induced replication (MMBIR), have been proposed.
Michael Krauthammer et al.
Nature genetics, 47(9), 996-1002 (2015-07-28)
We report on whole-exome sequencing (WES) of 213 melanomas. Our analysis established NF1, encoding a negative regulator of RAS, as the third most frequently mutated gene in melanoma, after BRAF and NRAS. Inactivating NF1 mutations were present in 46% of

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