N2911
Monoclonal Anti-Nck-2 antibody produced in mouse
~2 mg/mL, clone 8.8, purified immunoglobulin, buffered aqueous solution
Synonym(s):
Anti-Nck-β
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About This Item
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
8.8, monoclonal
form
buffered aqueous solution
mol wt
antigen 43 kDa
species reactivity
rat, chicken, rabbit, human
concentration
~2 mg/mL
technique(s)
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 1-2 μg/mL using rat tongue extract
isotype
IgG2b
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... NCK2(8440)
rat ... Nck2(316369)
General description
Monoclonal Anti-Nck-2 (mouse IgG2b isotype) is derived from the 8.8 hybridoma produced by the fusion of mice myeloma cells and splenocytes from BALB/c mice immunized with a C-terminal region of human Nck-2 protein fused to glutathione S-transferase (GST). NCK adaptor protein 2 (Nck-2) is a 43 kDa protein, that is 68 % identical at the protein level as that of Nck-1.
Nck-2 is an adaptor protein that regulates signal transduction in cells. This protein consists of one C-terminal SH2 domain and three N-terminal SH3 domains. Nck-2 interacts with several growth factor signaling components such as EGFR, IRS-1 and PDGFR, and also with the integrin-linked kinase signaling protein, LINCH .
Specificity
The epitope recognized by the antibody resides within amino acids 176-274 of human Nck-2. Cross reactivity has been observed with human Nck-1. Monoclonal Anti-Nck-2 is specific for human, rabbit, rat and chicken Nck-2 (approx. 43 kDa). The product also binds to human Nck-1.
Immunogen
C-terminal region of human Nck-2 protein (amino acids 115-380) fused to GST
Application
Monoclonal Anti-Nck-2 antibody produced in mouse has been used in:
- enzyme-linked immunosorbent assay (ELISA)
- immunoblotting
- immunoprecipitation
- immunocytochemistry
Biochem/physiol Actions
NCK adaptor protein 2 (Nck-2) can interact with kinases like the focal adhesion kinase (FAK), which is a cytoplasmic tyrosine kinase involved in the cellular control of motility. It stimulates human melanoma cell proliferation.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Find documentation for the products that you have recently purchased in the Document Library.
Nck adapter proteins: functional versatility in T cells
Lettau M, et al.
Cell communication and signaling : CCS, 7(1), 1-1 (2009)
Nck2 promotes human melanoma cell proliferation, migration and invasion in vitro and primary melanoma-derived tumor growth in vivo
Labelle-CM, et al.
BMC Cancer, 11(1), 443-443 (2011)
Nck-2 interacts with focal adhesion kinase and modulates cell motility
Goicoechea SM, et al.
The International Journal of Biochemistry & Cell Biology, 34(7), 791-805 (2002)
Y Tu et al.
Molecular biology of the cell, 9(12), 3367-3382 (1998-12-08)
Many of the protein-protein interactions that are essential for eukaryotic intracellular signal transduction are mediated by protein binding modules including SH2, SH3, and LIM domains. Nck is a SH3- and SH2-containing adaptor protein implicated in coordinating various signaling pathways, including
Michael C Brown et al.
Molecular biology of the cell, 16(9), 4316-4328 (2005-07-08)
The ArfGAP paxillin kinase linker (PKL)/G protein-coupled receptor kinase-interacting protein (GIT)2 has been implicated in regulating cell spreading and motility through its transient recruitment of the p21-activated kinase (PAK) to focal adhesions. The Nck-PAK-PIX-PKL protein complex is recruited to focal
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