N2786
Monoclonal Anti-Nedd8 antibody produced in mouse
clone ND23, purified immunoglobulin, buffered aqueous solution
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About This Item
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
ND23, monoclonal
form
buffered aqueous solution
species reactivity
human
technique(s)
microarray: suitable
western blot: 0.15-0.3 μg/mL using recombinant GST-Nedd8
isotype
IgG2b
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... NEDD8(4738)
General description
Monoclonal Anti-Nedd8 (mouse IgG2b isotype) is derived from the ND23 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to amino acids of human Nedd8 conjugated keyhole limpet hemocyanin (KLH). Nedd8 is a small protein (9 kDa) that shows the highest similarity to the ubiquitin protein (60% amino acid sequence identity).
Neural precursor cell expressed, developmentally down-regulated 8 (NEDD8) is an ubiquitin-like, 76 amino acid protein. It is expressed in endocrine and epithelial cells. NEDD8 belongs to the ubiquitin-like protein superfamily.
Immunogen
synthetic peptide corresponding to amino acids 20-32 of human Nedd8, conjugated to KLH.
Application
Monoclonal Anti-Nedd8 antibody produced in mouse has been used in:
- enzyme-linked immunosorbent assay (ELISA)
- immunoblotting
- immunocytochemistry
Biochem/physiol Actions
Nedd8 conjugation has a physiological role in processes where the stability of nuclear factor kappa light chain enhancer of activated B cells (NF-κB) and p27 is important.
Neural precursor cell expressed, developmentally down-regulated 8 (NEDD8) is involved in pathways concerned with protein degradation. It targets substrates for neddylation, which is a post-translational modification. NEDD8 also regulates the signaling of proteins like cullins.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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MLN4924, an NAE inhibitor, suppresses AKT and mTOR signaling via upregulation of REDD1 in human myeloma cells
Gu Y, et al.
Blood, 123(21), 3269-3276 (2014)
Angela Lucariello et al.
In vivo (Athens, Greece), 27(4), 501-506 (2013-07-03)
Neural precursor cell-expressed, developmentally down-regulated-8 (NEDD8) is a 76-amino-acid ubiquitin-like polypeptide. NEDD8 affects the signaling of various molecules but the major cellular target proteins are cullins. The neddylation process is correlated closely with apoptosis, cell-cycle regulation, embryogenesis and development. The
Marihan Hegazy et al.
Current protocols in cell biology, 89(1), e115-e115 (2020-10-13)
Biochemical methods can reveal stable protein-protein interactions occurring within cells, but the ability to observe transient events and to visualize the subcellular localization of protein-protein interactions in cells and tissues in situ provides important additional information. The Proximity Ligation Assay®
Yanchun Wang et al.
Cancer biology & therapy, 16(3), 420-429 (2015-03-18)
Recent studies indicate that post-translational protein neddylation is required for the maintenance of cell viability in several lymphoma cell lines, while inhibition of the neddylation pathway with an NEDD8-activating enzyme (NAE) inhibitor MLN4924 induces apoptosis in lymphoma cells. However, the
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