N0379
Nucleoside 5′-Diphosphate Kinase from baker′s yeast (S. cerevisiae)
lyophilized powder
Synonym(s):
ATP:Nucleoside diphosphate phosphotransferase, NDPK
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About This Item
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form
lyophilized powder
specific activity
1000-2000 units/mg protein
composition
Protein, 10-40% modified Warburg-Christian
UniProt accession no.
storage temp.
−20°C
Gene Information
bakers yeast ... YNK1(853798)
General description
Nucleoside 5′-diphosphate kinase is a cytosolic enzyme. [1]
Application
Biochem/physiol Actions
Nucleoside 5′-diphosphate kinase from Saccharomyces cerevisiae is found highly expressed in the cytoplasm. It affects DNA synthesis, in part, by binding to Cdc8p. [3]
Transfers one phosphate from many nucleoside triphosphates to many nucleoside diphosphates. No activity when UMP is substituted for the diphosphate.
Unit Definition
One unit will convert 1.0 μmole each of TDP and ATP to TTP and ADP per min at pH 7.6 at 25 °C in a coupled system with PK/LDH.
Physical form
Lyophilized, essentially sulfate-free powder. Contains sodium citrate with a trace of magnesium and EDTA salts.
Reconstitution
Reconstitute with deionized water. Solution is believed to be stable for over 1 year while frozen.
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Temporal and spatial distributions of yeast nucleoside diphosphate kinase activities and its association with the Cdc8p.
Zhang, SQ.
Cellular and Molecular Biology, 41, 333-346 (1995)
Takumi Chinen et al.
Nature communications, 6, 8722-8722 (2015-10-28)
Inhibitors of microtubule (MT) assembly or dynamics that target α/β-tubulin are widely exploited in cancer therapy and biological research. However, specific inhibitors of the MT nucleator γ-tubulin that would allow testing temporal functions of γ-tubulin during the cell cycle are
R Roy et al.
Biochemical and biophysical research communications, 122(3), 1418-1425 (1984-08-16)
Under standard conditions, in the presence of GTP, highly purified eIF-2 and Co-eIF-2 factor preparations efficiently stimulated AUG-codon dependent but not physiological mRNA-dependent Met-tRNAf binding to 40S ribosomes. Replacement of GTP by a nonhydrolyzable GTP analog, GMP-PNP, in the above
S Hemmerich et al.
Biochemistry, 30(6), 1523-1532 (1991-02-12)
Type I Fc epsilon receptor (Fc epsilon RI) mediated Ca2+ uptake and secretion of rat serosal mast cells have been shown to be inhibited by disodium 1,3-bis [(2'-carboxylatochromon-5'-yl) oxy]-2-hydroxypropane (disodium cromoglycate, DSCG), which is widely employed in the treatment of
J A Stahl et al.
Cancer research, 51(1), 445-449 (1991-01-01)
Reduced RNA and/or protein levels corresponding to the murine nm23-1 and human nm23-H1 complementary DNA clones have been correlated with high tumor metastatic potential in several rodent model systems and human breast carcinomas. We report the identification of a second
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