MX647S100

Mix-n-Stain^™ CF^™ 647 Antibody Labeling Kit (50-100μg)

• Synonym(s): Antibody Labeling Kit, CF 647 Labeling Kit, Mix-n-Stain Kit
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• form: liquid
• Quality Level: 100
• storage condition: protect from light
• fluorescence: λ_ex 650 nm; λ_em 665 nm
• storage temp.: −20°C

Description

General description

This kit contains everything you need to rapidly label an antibody. The labeling procedure comprises simple mixing of your antibody and the optimally formulated dye in the reaction buffer provided, followed by a brief incubation. The resulting solution is ready for staining without further purification. The kit is suitable for labeling commercially available primary antibodies, either directly or after a simple antibody clean-up step. After labeling, the dye is covalently linked to the antibody.

This labeling kit is for labeling 50-100 μg antibody with the far red fluorescent dye CF647. Mix-n-Stain CF dye antibody labeling kits are a revolutionary antibody labeling technology that permits one to label antibodies with our superior CF dyes in 30 minutes without a purification step. The labeling procedure tolerates many common buffer components and antibody stabilizers. CF647 is far-red fluorescent dye and one of an outstanding series of CF dyes. CF647 is spectrally similar to Alexa Fluor^™ 647 and Cy^®5 dyes. CF dyes are superior to Alexa Fluor^™ dyes and Cy^® dyes for antibody labeling by having combined advantages in brightness, photostability, specificity and novel features ideal for in vivo imaging.

Application

The Mix-n-Stain^™ CF^™ dye antibody labeling kit can be used to label primary antibodies[1][2] for ELISA, western blot, immunohistochemistry, flow cytometry, FACS,[3] immunofluorescence or immunocytochemistry applications.

Features and Benefits

A major advantage of the labeling kits is the superior CF dyes, many of which are brighter and more photostable than Alexa Fluor^™ dyes and other similar commercial dyes. With a large list of CF dyes of wavelengths covering the entire visible and near-infrared spectra, you have unprecedented freedom to label any commercial primary antibody with a color of your choice, making multi-color detection simple and affordable.

Mix-n-Stain CF dye antibody labeling kits dramatically simplify the process of preparing fluorescently labeled antibodies, particularly primary antibodies. Simply mix your antibody with a CF dye of your choice in the buffer provided, a step that takes less than 30 seconds of hands-on time. After waiting for another 30 minutes, without a separation step, you will have a fluorescent antibody conjugate that is as good as the best pre-labeled fluorescent antibody you can buy from some of the leading suppliers. There is no need to calculate how much dye you should use; just mix your antibody with the entire amount of dye provided and you will always produce optimal labeling. Moreover, the labeling reaction can tolerate the presence of common stabilizers, such as sodium azide, Tris, glycerol and low level of BSA.

Components

The kit consists of 1 vial each of Mix-n-Stain Dye, Mix-n-Stain Reaction Buffer (10x), Mix-n-Stain Storage Buffer, and an ultrafiltration vial.

Analysis Note

Direct replacement for: Alexa Fluor^™ 647, Cy^®5, DY- 647, DyLight^® 649, IRDye^® 650

Legal Information

This product is distributed by Sigma-Aldrich Co. under the authorization of Biotium, Inc. This product is covered by one or more US patents and corresponding patent claims outside the US patents or pending applications owned or licensed by Biotium, Inc. including without limitation: 12/334,387; 12/607,915; 12/699,778; 12/850,578; 61/454,484. In consideration of the purchase price paid by the buyer, the buyer is hereby granted a limited, non-exclusive, non-transferable license to use only the purchased amount of the product solely for the buyer′s own internal research in a manner consistent with the accompanying product literature. Except as expressly granted herein, the sale of this product does not grant to or convey upon the buyer any license, expressly, by implication or estoppel, under any patent right or other intellectual property right of Biotium, Inc. Buyer shall not resell or transfer this product to any third party, or use the product for any commercial purposes, including without limitation, any diagnostic, therapeutic or prophylactic uses. This product is for research use only. Any other uses, including diagnostic uses, require a separate license from Biotium, Inc. For information on purchasing a license to use this product for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: [email protected].

ALEXA FLUOR is a trademark of Life Technologies

CF is a trademark of Biotium, Inc.

Cy is a registered trademark of Cytiva

DyLight is a registered trademark of Pierce Biotechnology, Inc.

IRDye is a registered trademark of LI-COR, Inc.

Mix-n-Stain is a trademark of Biotium, Inc.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• pictograms: GHS05
• signalword: Warning
• hcodes: H290,H412
• pcodes: P234 - P273 - P390 - P501
• Hazard Classifications: Aquatic Chronic 3 - Met. Corr. 1
• Storage Class: 8A - Combustible corrosive hazardous materials
• flash_point_f: Not applicable
• flash_point_c: Not applicable