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MITOISO2

Sigma-Aldrich

Mitochondria Isolation Kit

sufficient for 50 applications (2-5 x 107 cells), isolation of enriched mitochondrial fraction from cells

Synonym(s):

Mitochondrial Extraction Kit, Mitochondrial Purification Kit

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

Quality Level

200

usage

sufficient for 50 applications (2-5 x 107 cells)

technique(s)

ELISA: suitable
western blot: suitable

shipped in

dry ice

storage temp.

−20°C

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General description

Mitochondria, a double membrane structure, is the site of most energy production in eukaryotic cells. The mitochondria isolation kit contains two buffers: one for extracting proteins for functional studies and another for profiling in proteome studies. Additionally, the kit includes a storage buffer for preserving intact mitochondria.

Application

Mitochondria Isolation Kit has been used in the isolation of mitochondria from:
  • Ewing′s Sarcoma Family of Tumor (ESFT) cells
  • blood samples of tuberculosis patients
  • human neuroblastoma cells and glioma cells
  • myotubes
  • HepG2 cells

Features and Benefits

  • The kit allows for quick and simple extraction of a concentrated portion of mitochondria from cells.
  • The isolated mitochondria will mostly have intact inner and outer membranes.
  • Can be used to extract mitochondrial proteins for proteome analysis

Other Notes

  • This product is for R&D use only, not for drug, household, or other uses.
  • Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.
  • Use ultrapure water for the preparation of reagents.
  • Please refer to the product information sheet to learn more about kit components, reagents required, storage and stability, and isolation protocols.

Kit Components Also Available Separately

Product No.
Description
SDS
  • C2978CelLytic™ M, Cell Lysis Reagent, Suitable for Mammalian cell lysis and protein solubilization. 10 mLSDS
  • P8340Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution 2.5 mLSDS
  • C0356Protein Extraction Reagent Type 4 1 bottleSDS

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Warning

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2

Storage Class

10 - Combustible liquids

flash_point_f

188.6 °F - closed cup

flash_point_c

87 °C - closed cup

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Imran Khan et al.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 84, 789-799 (2016-10-11)
Although quercetin is very well known for its anticancer activity, however it shows some drawbacks. Herein, we have evaluated the apoptotic effect TEF (5, 3'-dihydroxy-3, 7, 4'-triethoxyflavone), a newly synthesized quercetin derivative on HCT-116 colon cancer cells. After 24h of
Detection and characterisation of multi-drug resistance protein 1 (MRP-1) in human mitochondria
Roundhill, EA and Burchill, SA
British Journal of Cancer, 106(6), 1224-1224 (2012)
Linezolid trough concentrations correlate with mitochondrial toxicity-related adverse events in the treatment of chronic extensively drug-resistant tuberculosis
Song T, et al.
EBioMedicine, 2(11), 1627-1633 (2015)
Effects of Moringa oleifera Leaves Extract on High Glucose-Induced Metabolic Changes in HepG2 Cells
Sosa-Gutierrez J, et al.
Biology, 7(3), 37-37 (2018)
Mitochondria
Cooper G M
Journal of biological and chemical chronicles (2000)
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Cellular oxidative stress is countered by enzymatic scavengers and antioxidant modulators against reactive oxygen species damage.

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