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MAK344

Sigma-Aldrich

Cell Cycle Analysis Kit

sufficient for 100 assays

Synonym(s):

Cell Cycle Staining Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 100 assays

detection method

fluorometric

relevant disease(s)

cancer

storage temp.

−20°C

General description

Cell cycle is a ubiquitous, complex sequence of events leading to growth and proliferation of cells. Cell cycle progression is tightly regulated due to its involvement in development, DNA damage and repair, etc. Anomalies in cell cycle progression can lead to tissue hyperplasia and diseases such as cancer. Cell cycle can be subdivided into interphase (G0/G1, S and G2) and mitotic (M) phase (prophase, metaphase, anaphase and telophase).

Application


  • HMGB2 Upregulation Promotes the Progression of Hepatocellular Carcinoma Cells through the Activation of ZEB1/vimentin Axis.: This research employed cell cycle analysis to elucidate the role of HMGB2 in hepatocellular carcinoma. The findings highlighted how HMGB2 influences cell cycle dynamics and metastatic properties, emphasizing the kit′s application in cancer research (Lu et al., 2023).

  • Exploring the Anti-Glioma Mechanism of the Active Components of Cortex Periplocae Based on Network Pharmacology and iTRAQ Proteomics In Vitro.: This study integrated cell cycle analysis with network pharmacology and iTRAQ proteomics to uncover the anti-glioma properties of Cortex Periplocae components. It demonstrated the comprehensive application of the kit in neuro-oncology research (Huan et al., 2023).

  • ERH Gene Knockdown Inhibits the Proliferation and Migration of ARPE-19 Cells through MCM Complex and EMT Process.: Using a cell cycle analysis kit, this research identified the impact of ERH gene knockdown on cell proliferation and migration in ARPE-19 cells. The study underscored the kit′s role in gene function studies and cell biology (Li et al., 2023).

  • Quantitative Proteomics Reveals Effects of Environmental Radiofrequency Electromagnetic Fields on Embryonic Neural Stem Cells.: This investigation used cell cycle analysis to study the effects of radiofrequency electromagnetic fields on embryonic neural stem cells. The findings provided valuable insights into environmental impacts on cell cycle and stem cell biology (An et al., 2023).

Features and Benefits

The method is non-radioactive, rapid and accurate and can be used for high throughput cell cycle analysis with contemporary flow cytometer instruments.

Suitability

Suitable for studying cell cycle in adherent or suspension cells

Principle

The kit utilizes a nuclear dye, the binding of which to nucleic acids in the cell results in fluorescence signal, which is proportional to cellular DNA content. The percentages of cells in different phases of the cell cycle (G0/G1, S, and G2/M) can be quantified by flow cytometry. 

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
8J23K09200
8B17K09200
7H05K09200
7B16K09200 
7B16K09200

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Questions

1–2 of 2 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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