MAK326
Indole Assay Kit
sufficient for 100 colorimetric tests
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About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
Recommended Products
usage
sufficient for 100 colorimetric tests
application(s)
cosmetics
food and beverages
detection method
colorimetric
relevant disease(s)
gastrointestinal diseases
storage temp.
2-8°C
General description
Indole is the primary product of tryptophan breakdown by tryptophanase. Structurally, it is characterized by a benzene ring fused to a pyrrole ring, containing a five-membered nitrogen-containing ring. This simple structure forms the core of a vast array of natural and synthetic compounds with a wide range of biological activities. In bacteria, indole is a critical signaling molecule, produced as a metabolite during the breakdown of the amino acid tryptophan. It plays a pivotal role in bacterial behavior, influencing biofilm formation, spore germination, and the expression of virulence factors. In the context of metabolism in larger organisms, indole derivatives are integral to the functioning of the central nervous system, serving as precursors for the synthesis of neurotransmitters such as serotonin and melatonin. These compounds are consequential in regulating mood, appetite, sleep, and circadian rhythms. Indole metabolism in the gut microbiota has been implicated in maintaining the integrity of the intestinal epithelial barrier and modulating the immune response, reflecting the complexity and importance of this molecule in biological systems.
Indole is the primary product of tryptophan breakdown by tryptophanase. The indole test is commonly performed on bacteria to classify them on their ability to break down tryptophan to indole.
Application
The Nitric Oxide Assay Kit may be used for:
- Gastrointestinal Disease Research
- Bacterial Culture Analysis
Features and Benefits
Quick and Sensitive: Detect Indole levels ranging from 3 - 100 μM with a short 10 minute procedure, providing both convenience and sensitivity.
Simplified Process: Enjoy a hassle-free procedure that requires the addition of a single working reagent, saving you time and effort.
Simplified Process: Enjoy a hassle-free procedure that requires the addition of a single working reagent, saving you time and effort.
Suitability
Suitable for indole determination in biological samples (e.g. indole produced by indole positive bacteria).
Principle
The indole assay kit is based on a modified version of Ehlrich′s and Kovac′s reagents, which reacts with indole to produce a colored compound at 565 nm. The intensity of this colored compound is directly proportional to the indole in the sample. Linear detection range from 3 to 100 μM indole in 96-well plate assay.
Other Notes
For additional information on our range of Biochemicals, please complete this form.
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Danger
Hazard Classifications
Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3
target_organs
Central nervous system, Respiratory system
Storage Class
3 - Flammable liquids
Certificates of Analysis (COA)
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