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MAK293

Sigma-Aldrich

Collagenase Activity Colorimetric Assay Kit

Collagenase Activity Colorimetric Assay Kit

sufficient for 100 colorimetric tests

Synonym(s):

Collagenase Detection Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.25

detection method

colorimetric

relevant disease(s)

cancer

storage temp.

−20°C

General description

Collagenase (EC 3.4.24.3) is an enzyme in the matrix metalloproteinase family that breaks down collagen, assisting in degradation of the extracellular matrix, a key step in the pathogenesis of bacteria. Collagen is an abundant structural protein present in the connective tissue of animals. Collagenase has been used clinically for the treatment of Dupuytren′s contracture, an affliction characterized by a thickening of connective tissue.

Suitability

Suitable for the measurement of collagenase activity in bacterial extract and for screening/studying/characterizing collagenase inhibitors in purified enzyme.

Principle

This Collagenase Activity Assay Kit provides a quick and easy way to determine activity of collagenase. The kit measures collagenase activity using a synthetic peptide (FALGPA) that mimics collagen′s structure. It is suitable for measuring activity of bacterial collagenases such as from Clostridium histolyticum. In addition, it can also be used to screen/characterize collagenase inhibitors. The limit of detection for this assay is 0.02 mU of collagenase.

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Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Irrit. 2 - Flam. Liq. 2

Storage Class

3 - Flammable liquids

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Certificates of Analysis (COA)

Lot/Batch Number
8G12K07920
8F03K07920
8B16K07920
7M13K07920
7G20K07920

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1 Review This action will navigate to reviews.

Questions

1–2 of 2 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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Reviews

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    • Review 1
    • Votes 0
    2 out of 5 stars.

    Review

    The product advertise 100 reactions, but the reality is much less if you consider that you need to include chemical controls, background, run your samples in duplicate (at least).

    Helpful?

    1. Response from MilliporeSigma:

      Thank you for reaching out and sharing your review with us. Unfortunately, the protocol does recommend samples and standards be run in duplicate. This is fairly common and ensures accuracy of results. Using a smaller format such as a 384-well plate may be an option should you need to maximize your kit, however it has only been validated using the 96-well format. We would like to learn more about your experience. When you have a moment, please contact us by visiting https://www.sigmaaldrich.com/support/customer-support and submitting a Product Technical Inquiry. We hope to hear from you soon!

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