MAK283
Myeloperoxidase (MPO) Inhibitor Screening Kit (Fluorometric)
sufficient for 200 fluorometric tests
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About This Item
UNSPSC Code:
12352200
Recommended Products
detection method
fluorometric
relevant disease(s)
cardiovascular diseases; cancer
storage temp.
−20°C
General description
Myeloperoxidase (MPO: EC 1.11.2.2) is a peroxidase abundantly expressed in neutrophil granulocytes. It catalyzes the hydrogen peroxide dependent oxidation of halide ions to generate hypochlorite (HClO), the reaction by which MPO exhibits cytotoxic activity against tumor cells and microorganisms. MPO also oxidizes various substances such as phenol and anilines. MPO undergoes chlorination or peroxidation reaction depending upon the relative concentrations of chloride and the reducing substrates. Recent studies suggest that increased levels of MPO are associated with an increased risk for cardiovascular disease and myocardial infarction, thus development of novel and specific inhibitors of MPO is critical for therapeutic purposes.
Features and Benefits
Compatible with high-throughput handling systems.
Suitability
Suitable for screening/characterizing/studying potential inhibitors of Myeloperoxidase
Principle
This MPO Inhibitor Screening Kit provides screening assays for both MPO chlorination and peroxidation activities. In the chlorination assay (specific for MPO), MPO converts hydrogen peroxide and sodium chloride to sodium hypochlorite, which reacts with the chlorination substrate to give an intensely fluorescent product, ex = 480 nm/em = 520. In the peroxidation assay, MPO oxidizes peroxidation substrate to generate fluorescence ex = 535 nm/em = 587. The fluorescence generated is directly proportional to any peroxidase activity present. In the presence of MPO inhibitor, reactions are impeded, thus decreasing the rate and/or extent of generation of MPO-dependent fluorescence.
Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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