MAK236
Factor VIIIa Activity Assay Kit (Fluorometric)
sufficient for 100 fluorometric tests
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About This Item
UNSPSC Code:
12352200
Recommended Products
detection method
fluorometric
relevant disease(s)
hematological disorder
storage temp.
−20°C
General description
The coagulation Factor VIII, also known as anti-hemophilic factor (AHF) is a vital blood-clotting protein. Factor VIII circulates in the bloodstream as an inactive protein, bound to a large multimeric glycoprotein called von Willebrand factor (VWF). Upon an injury to the blood vessel, VWF dissociates from FVIII and releases the active form of FVIII (FVIIIa). In the presence of calcium ions and negatively charged membrane phospholipids, activated factor VIII (FVIIIa) then binds to the activated Factor IX (FIXa) and proteolytically activates factor X (FX) to factor Xa (FXa).
Acquired factor VIII deficiency results in skin, muscle and tissue hemorrhage.[1]
Acquired factor VIII deficiency results in skin, muscle and tissue hemorrhage.[1]
Suitability
Suitable for the determination of Factor VIII activity in serum and plasma samples
Principle
This Factor VIIIa Activity Assay kit is based on the ability of FVIIIa to generate FXa. The generated FXa proteolytically cleaves a synthetic substrate and releases a fluorophore, AMC, which can be easily quantified by a fluorescence microplate reader. The assay is simple, rapid, and can detect activity as low as 1 ng of FVIIIa in a variety of samples.
Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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Autoantibodies to coagulation factors: from pathophysiology to diagnosis and therapy
Cugno M, et al.
Autoimmunity Reviews, 13(1), 40-48 (2014)
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