MAK235
Adenylate Kinase Activity Assay Kit (Colorimetric/Fluorometric)
sufficient for 100 colorimetric or fluorometric tests
Synonym(s):
Adenylate Kinase Test Kit
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About This Item
UNSPSC Code:
12352200
Recommended Products
detection method
colorimetric
fluorometric
relevant disease(s)
hematological disorder; cardiovascular diseases; cancer
storage temp.
−20°C
General description
Adenylate Kinase (AK) (EC 2.7.4.3) is an abundant enzyme involved in energy metabolism and homeostasis of cellular adenine nucleotide ratios in different intracellular compartments. The enzyme is found in the nucleus, cytosol, or mitochondria (intermembrane space or matrix) of various kinds of tissues. Adenylate kinase acts on two molecules of ADP to generate ATP and AMP. Nine isoforms of adenylate kinase have been identified. Erythrocyte adenylate kinase deficiency is associated with hemolytic anemia. Adenylate kinase also plays an important role in post-ischemic recovery and in apoptosis.
About 30 – 40% of adenylate kinase activity takes place in mitochondria of heart muscles.[1] Mutation in adenylate kinase gene is associated with a severe combined immunodeficiency referred as reticular dysgenesis.[2]
About 30 – 40% of adenylate kinase activity takes place in mitochondria of heart muscles.[1] Mutation in adenylate kinase gene is associated with a severe combined immunodeficiency referred as reticular dysgenesis.[2]
Suitability
Suitable for the detection of Adenylate Kinase in cell/tissue homogenate, mitochondrial lysate, or purified protein.
Principle
This AK Activity Assay kit can kinetically measure Adenylate Kinase activity by detecting adenosine triphosphate (ATP) generated from adenosine diphosphate (ADP) as a substrate. ATP is detected via a multi-step reaction, resulting in the generation of an intermediate that reacts with the Adenylate Kinase Probe forming a colorimetric (570 nm)/fluorometric (ex = 535 nm/em = 587 nm) product.
signalword
Danger
Hazard Classifications
Acute Tox. 4 Dermal - Aquatic Chronic 3 - Eye Dam. 1 - Repr. 2 - Resp. Sens. 1 - Skin Sens. 1
Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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Adenylate kinase and AMP signaling networks: metabolic monitoring, signal communication and body energy sensing.
Dzeja P and Terzic A
International Journal of Molecular Sciences, 10(4) (2009)
Molecular Basis Of Congenital Neutropenia
Klein C.
The Hematology Journal : The Official Journal of the European Haematology Association / EHA (2009)
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