The product MAK219 is a simple yet sensitive assay that can detect normal and abnormal concentrations of Tyr in biological fluids. It has been tested in serum, plasma, urine, and other body fluids, but not in other samples. Notably, it does not differentiate between free and bound tyrosine.
MAK219
Tyrosine Colorimetric Assay Kit
Sufficient for 100 Colorimetric tests
Synonym(s):
Tyrosine Detection Colorimetric Kit
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About This Item
Recommended Products
detection method
colorimetric
relevant disease(s)
neurological disorders
storage temp.
−20°C
General description
Tyrosine (Tyr) is an aromatic amino acid that is a precursor of several important substrates such as catecholamine, thyroid hormone, and melanin pigment. A deficiency in enzymes such as homogentisic acid oxidase or phenylalanine 4-hydroxylase that degrade tyrosine result in rare, hereditary metabolic disorders.
Suitability
Suitable for the detection of tyrosine in a variety of biological samples.
Principle
The Tyrosine Colorimetric Assay Kit provides a simple assay for measuring tyrosine in biological samples (ranging from 5-75 nmole/well). Tyrosine is determined by measuring a colorimetric product with absorbance at 492 nm (A492) proportional to the amount of tyrosine present.
Kit Components Only
Product No.
Description
- Tyr Assay Buffer
- Tyr Enzyme Mix
- Tyr Standard
signalword
Danger
hcodes
Hazard Classifications
Eye Dam. 1 - Resp. Sens. 1
Storage Class
10 - Combustible liquids
wgk_germany
WGK 3
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Li Mao et al.
Oncotarget, 8(59), 99681-99692 (2017-12-17)
MicroRNAs (miRNAs, miR) play a fundamental role in cerebral ischemia/reperfusion (I/R) injury. However, the role of miRNAs in toxic aldehyde and tyrosine accumulation is not fully elucidated. We constructed a cerebral I/R rat model and found that overexpression of miR-193
Li-Mao et al.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 84, 1923-1929 (2016-11-17)
To explore the new mechanism of neuroprtection of monosialotetrahexosylganglioside and providing reliable theoretical foundation and experimental evidence for the emergency treatment and rehabilitation of cerebral ischemia/reperfusion injury. A rat model of cerebral ischemia/reperfusion injury was constructed and intervened with monosialotetrahexosylganglioside(5mg/kg)
Delong Jiao et al.
Bioactive materials, 6(7), 2011-2028 (2021-01-12)
Developmental engineering strategy needs the biomimetic composites that can integrate the progenitor cells, biomaterial matrices and bioactive signals to mimic the natural bone healing process for faster healing and reconstruction of segmental bone defects. We prepared the gelatin-reduced graphene oxide
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Can the assay detect bound tyrosine, such as tyrosine in a protein, or is the assay only capable of detecting free, unbound tyrosine in solution?
1 answer-
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How cell culture samples should be prepared for the product MAK219?
1 answer-
According to the vendor, the preparation of cell culture samples for the product MAK219 has not been specifically tested, and thus, there is no corresponding protocol available. However, a general protocol for the lysis of tissue and cells for activity assays is provided, which can be adapted for the experiment. The protocol includes specific steps for the lysis of cultured cells, such as resuspending the cell pellets in the desired lysis buffer, sonication, centrifugation, and harvesting the supernatant for the assay. Additionally, information is provided on the suggested lysis buffer and an alternative lysis solution using PBS + 0.5% NP-40. It is advised to check the compatibility of the components to ensure they will not interfere with the assay.
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