The kit is sourced from an outside supplier and contains approximately 1 ug of Neutrophil Elastase (MAK213C) in the vial with an activity range of 15-25 micro units/vial. It's important to note that the activity of each lot is not required since it is an inhibition assay, and only the relative activity is determined in the calculation.
MAK213
Neutrophil Elastase Inhibitor Screening Kit
Sufficient for 100 Fluorometric tests
Synonym(s):
Neutrophil Elastase Inhibition Assay Kit
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About This Item
Recommended Products
detection method
fluorometric
storage temp.
−20°C
Gene Information
human ... ELANE(1991)
mouse ... ELANE(50701)
rat ... ELANE(299606)
General description
Neutrophil elastase (NE) is a serine protease that hydrolyzes proteins within the azurophilic granules of the neutrophils. Once secreted, it digests collagen-VI and elastin of the extracellular matrix. Neutrophil elastase is implicated in cystic fibrosis, bronchiectasis, chronic obstructive pulmonary disease, congenital neutropenia, and lung cancer.[1][2][3]
Features and Benefits
Compatible with high-throughput handling systems.
Suitability
Suitable for the screening of inhibitors of Neutrophil Elastase.
Principle
The Neutrophil Elastase Inhibitor Screening Kit is a rapid, sensitive and high throughput assay to screen and characterize potential inhibitors of NE. NE activity is measured by hydrolyzing the substrate to yield a fluorescent product (λex = 400/λem = 505 nm) proportional to the enzymatic activity present.
Kit Components Only
Product No.
Description
- Assay Buffer
- Substrate
- Neutrophil Elastase
- Inhibitor Control, 3 mM SPCK
Storage Class
10 - Combustible liquids
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Could you provide details on the content of the Neutrophil Elastase (MAK213C) included in the kit in terms of the quantity present? This is to ensure that the testing will involve similar NE concentrations. The kit instructs to reconstitute with 220uL but does not specify the final concentration.
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