MAK211
Lipolysis Colorimetric Assay Kit
Sufficient for 100 Colorimetric tests
Synonym(s):
Lipolysis Quantification Kit
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About This Item
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detection method
colorimetric
relevant disease(s)
endocrinological disorders, diabetes
storage temp.
−20°C
General description
Lipolysis is the process of hydrolyzing triglycerides to free fatty acids and glycerol. This process involves the action of adipose TG lipase (ATGL), hormone-sensitive lipase (HSL), and monoglyceride lipase. Lipolysis maintains the energy balance during fasting and exercise by providing a substrate for oxidative metabolism. Lipolysis is regulated by nutritional factors and hormones. Problems with the regulation of lipolysis is associated with obesity, diabetes, and metabolic syndromes.
Suitability
Suitable for the measurement of lipolysis in 3T3-L1 and adipocytes.
Principle
The Lipolysis (3T3-L1) Colorimetric Assay Kit contains a synthetic catecholamine (isoproterenol) that activates β-adrenergic receptors. This activates adenylate cyclase, converting ATP to cAMP. cAMP then activates the hydrolysis of triglycerides by hormone-sensitive lipase. Lipolysis is determined by measuring a colorimetric product with absorbance at 570 nm (A570) proportional to the amount of glycerol present.
Kit Components Only
Product No.
Description
- Lipolysis Assay Buffer
- Lipolysis Wash Buffer
- Glycerol Assay Buffer
- Glycerol Probe, in DMSO
- Glycerol Enzyme Mix
- Glycerol Standard, 100 mM
- Isoproterenol, 10 mM
replaced by
Product No.
Description
Pricing
signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class
10 - Combustible liquids
Certificates of Analysis (COA)
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Lipolysis?a highly regulated multi-enzyme complex mediates the catabolism of cellular fat stores.
Lass A, et al.
Progress in Lipid Research, 50(1), 14-27 (2011)
Control of fatty acid and glycerol release in adipose tissue lipolysis.
Langin D.
Comptes rendus biologies, 329(8), 598-607 (2006)
John M Dean et al.
Cell reports, 33(1), 108228-108228 (2020-10-08)
The Mediator complex relays regulatory signals from gene-specific transcription factors to the basal transcriptional machinery. However, the role of individual Mediator subunits in different tissues remains unclear. Here, we demonstrate that MED19 is essential for adipogenesis and maintenance of white
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