The use of RIPA or other harsh cell lysis buffers is not recommended, as they may denature and/or inhibit enzymes that are crucial for the kit's performance. According to the protocol of MAK207, tissue samples (10 mg) can be homogenized in 100 mL of ice-cold G3P Assay Buffer (MAK207A in the kit), kept on ice for 10 minutes, centrifuged at 12,000 x g for 5 minutes to remove insoluble material, and then transferred to a fresh tube.
MAK207
Glycerol-3-Phosphate Assay Kit
Sufficient for 100 Colorimetric tests
Synonym(s):
G3P Assay Kit
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About This Item
Recommended Products
detection method
colorimetric
relevant disease(s)
gastrointestinal diseases
storage temp.
−20°C
General description
Glycerol 3-phosphate (G3P) is an important intermediate of carbohydrate and lipid metabolic pathways. It is produced from glycerol by glycerol kinase or from dihydroxyacetone phosphate by glycerol 3-phosphate dehydrogenase. G3P may enter the G3P shuttle to generate NAD+, or may be converted to glyceraldehyde 3-phosphate and enter glycolysis or the lipid biosynthesis pathway.
Application
Suitability
Suitable for the detection of Glycerol-3-Phosphate in tissue and cell culture samples.
Principle
The Glycerol 3-Phosphate Colorimetric Assay Kit provides a simple assay for measuring G3P in various tissues and cells (ranging from 2-10 nmole/well). G3P is determined by measuring a colorimetric product with absorbance at 450 nm (A450) proportional to the amount of G3P present.
Kit Components Only
Product No.
Description
- G3P Assay Buffer
- G3P Enzyme Mix
- G3P Probe
- G3P Standard
replaced by
Product No.
Description
Pricing
signalword
Danger
hcodes
Hazard Classifications
Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B
Storage Class
8A - Combustible corrosive hazardous materials
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