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MAK183

Sigma-Aldrich

PDH Activity Assay Kit

Sufficient for 100 Colorimetric tests

Synonym(s):

Pyruvate Dehydrogenase Activity Assay Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 100 colorimetric tests

detection method

colorimetric

relevant disease(s)

neurological disorders; cancer

storage temp.

−20°C

Gene Information

human ... PDHA1(5160) , PDHA2(5161) , PDHB(5162) , PDHX(8050)
mouse ... PDHA1(18597) , PDHA2(18598) , PDHB(68263) , PDHX(27402)
rat ... PDHA1(29554) , PDHA2(117098) , PDHB(289950) , PDHX(311254)

General description

Pyruvate dehydrogenase (PDH) is a mitochondrial enzyme that catalyzes the conversion of pyruvate to acetyl-CoA and CO2, and also links the tricarboxylic acid (TCA) and glycolysis pathways.[1] The enzyme is inhibited by phosphorylation and activated by dephosphorylation. Mutations in PDH have been linked to pyruvate dehydrogenase deficiency (causing lactic acidosis and neurologic dysfunctions) and Leigh syndrome.[2] PDH has also been implicated in oncogene-induced senescence.[3] PDH measurements can provide insights into metabolic functions and oncogenesis.

Application

PDH Activity Assay Kit has been used to determine the activity of pyruvate dehydrogenase.[4][5][6]

Suitability

Suitable for the detection of PDH activity in a variety of biological samples such as cell and tissue culture supernatants and purified mitochondria.

Principle

Pyruvate dehydrogenase activity is determined using a coupled enzyme reaction, which results in a colorimetric (450 nm) product proportional to the enzymatic activity present. One unit of pyruvate dehydrogenase is the amount of enzyme that will generate 1.0 mmole of NADH per minute at 37 °C.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Kit Components Only

Product No.
Description
  • PDH Assay Buffer
  • PDH Substrate
  • PDH Developer
  • NADH Standard
  • PDH Positive Control

pictograms

CorrosionExclamation mark
GHS05,GHS07

signalword

Danger

hcodes

H314,H317

Hazard Classifications

Eye Dam. 1 - Skin Corr. 1B - Skin Sens. 1

Storage Class

8A - Combustible corrosive hazardous materials

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Certificates of Analysis (COA)

Lot/Batch Number
8F02K06790
7F25K06790
3H22K06790
B2I090679V
B2F090679V

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The spectrum of pyruvate dehydrogenase complex deficiency: clinical, biochemical and genetic features in 371 patients.
Patel K P, et al.
Molecular Genetics and Metabolism, 106(3), 385-394 (2012)
Carine Lindquist et al.
PloS one, 14(9), e0222558-e0222558 (2019-09-25)
A fatty acid analogue, 2-(tridec-12-yn-1-ylthio)acetic acid (1-triple TTA), was previously shown to have hypolipidemic effects in rats by targeting mitochondrial activity predominantly in liver. This study aimed to determine if 1-triple TTA could influence carbohydrate metabolism. Male Wistar rats were
Pharmacological Blockade of Cannabinoid CB1 Receptors in Diet-Induced Obesity Regulates Mitochondrial Dihydrolipoamide Dehydrogenase in Muscle.
Arrabal S, et al
PLoS ONE, 10(12), e0145244-e0145244 (2015)
The Citric Acid Cycle Oxidizes Two-Carbon Units
Berg J M, et al.
Biochemistry (2002)
Minfeng Ying et al.
The Journal of biological chemistry, 294(24), 9615-9630 (2019-05-02)
Whether growing cancer cells prefer lactate as a fuel over glucose or vice versa is an important but controversial issue. Labeling of tricarboxylic acid (TCA) cycle intermediates with glucose or lactate isotope tracers is often used to report the relative
View All Related Papers

Questions

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  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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