MAK170
Glutamate Oxidase Assay Kit
sufficient for 200 fluorometric tests
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usage
sufficient for 200 fluorometric tests
detection method
fluorometric
relevant disease(s)
cancer
storage temp.
−20°C
General description
Glutamate oxidase is an oxidoreductase that catalyzes the oxidative deamination of L-glutamate to ketoglutarate, ammonia, and hydrogen peroxide.
The Glutamate Oxidase Assay Kit provides a simple and direct procedure for measuring glutamate oxidase activity in a variety of samples such as solutions and cell extracts. Glutamate Oxidase activity is determined by a coupled enzyme assay, in which the oxidation of glutamic acid results in a fluorescent product (λex =540/λem = 590 nm) proportional to the glutamate oxidase activity present. One unit of glutamate oxidase activity is defined as the amount of enzyme that catalyzes the conversion of L-glutamic acid to 1.0 μmole a-ketoglutaric acid per minute at pH 7.4 at room temperature.
The Glutamate Oxidase Assay Kit provides a simple and direct procedure for measuring glutamate oxidase activity in a variety of samples such as solutions and cell extracts. Glutamate Oxidase activity is determined by a coupled enzyme assay, in which the oxidation of glutamic acid results in a fluorescent product (λex =540/λem = 590 nm) proportional to the glutamate oxidase activity present. One unit of glutamate oxidase activity is defined as the amount of enzyme that catalyzes the conversion of L-glutamic acid to 1.0 μmole a-ketoglutaric acid per minute at pH 7.4 at room temperature.
Suitability
Suitable for measuring glutamate oxidase activity in a variety of samples such as solutions and cell extracts.
Principle
The glutamate oxidase assay kit provides a simple and direct procedure for measuring glutamate oxidase activity in a variety of samples such as solutions and cell extracts. Glutamate oxidase activity is determined by a coupled enzyme assay, in which the oxidation of glutamic acid results in a fluorescent product (λex =540/λem = 590 nm) proportional to the glutamate oxidase activity present. One unit of glutamate oxidase activity is defined as the amount of enzyme that catalyzes the conversion of L-glutamic acid to 1.0μmole α-ketoglutaric acid per minute at pH 7.4 at room temperature.
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Danger
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Resp. Sens. 1 - Skin Sens. 1
Storage Class
10 - Combustible liquids
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Purification and properties of a new enzyme, l-glutamate oxidase, from Streptomyces sp. X-119-6 grown on wheat bran.
Kusakabe H, et al.
Agricultural and Biological Chemistry, 47(6), 1323-1328 (1983)
Jiro Arima et al.
The FEBS journal, 276(14), 3894-3903 (2009-06-18)
L-Glutamate oxidase (LGOX) from Streptomyces sp. X-119-6, which catalyzes the oxidative deamination of L-glutamate, has attracted increasing attention as a component of amperometric L-glutamate sensors used in the food industry and clinical biochemistry. The precursor of LGOX, which has a
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