MAK064
Lactate Assay Kit
sufficient for 100 colorimetric or fluorometric tests
Synonym(s):
Lactic Acid Assay Kit
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About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
Recommended Products
usage
sufficient for 100 colorimetric or fluorometric tests
application(s)
cosmetics
food and beverages
detection method
colorimetric
fluorometric
relevant disease(s)
endocrinological disorders, diabetes; cancer
storage temp.
−20°C
General description
L(+)-Lactate is a metabolic compound formed in animals by the action of the enzyme Lactate Dehydrogenase. Lactate is produced in proliferating cells and during anaerobic conditions such as exercise. Abnormally high concentrations of lactate have been related to pathological conditions such as cancer, diabetes, and lactate acidosis. L(+)-Lactate is the major stereoisomer of lactate formed in human intermediary metabolism and is present in blood at levels of around 1-2 mmol/L. This kit provides a convenient means for detecting L(+)-Lactate in biological samples. There is no need of pretreatment or purification of samples.
Application
Lactate Assay Kit has been used to determine the concentrations of lactate in samples.
Suitability
Suitable for detecting L(+)-Lactate in culture medium, fermentation medium, blood and cells.
Principle
In this assay, lactate concentration is determined by an enzymatic assay, which results in a colorimetric (570 nm)/ fluorometric (λex = 535 nm/λem = 587 nm) product, proportional to the lactate present. Typical levels of sensitivity for this kit are between 0.001-10 mM.
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Danger
hcodes
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class
10 - Combustible liquids
flash_point_f
188.6 °F - closed cup
flash_point_c
87 °C - closed cup
Certificates of Analysis (COA)
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Min Xu et al.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 47(1), 151-160 (2018-05-16)
Poor response to chemotherapy leads to the relapse and metastatic progression of tumors. Reprogrammed glucose metabolism is one of the important hallmarks of cancer that facilitates cancer cell survival, proliferation and chemoresistance. However, the precise fate of glucose metabolism and
Juping Zhao et al.
Molecular medicine reports, 16(6), 8335-8344 (2017-10-07)
Previous studies have indicated that high expression of lactate dehydrogenase A (LDHA) exists in many human cancers. Recently, several reports showed that silencing or inhibition of LDHA could suppress metastasis of human cancer including renal cell carcinoma (RCC). However, the
Khalid N M Abdelazeem et al.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 41(6), 2374-2382 (2017-05-04)
Key properties of tumor cells include enhanced glycolytic flux with excessive consumption of glucose and formation of lactate. As glycolysis is highly sensitive to cytosolic pH, maintenance of glycolysis requires export of H+ ions, which is in part accomplished by
Prabha Shrestha et al.
PLoS pathogens, 13(9), e1006628-e1006628 (2017-09-19)
Primary effusion lymphoma (PEL) is an aggressive B-cell lymphoma with poor prognosis caused by Kaposi's sarcoma-associated herpesvirus (KSHV). Previous studies have revealed that HIF-1α, which mediates much of the cellular response to hypoxia, plays an important role in life cycle
Bibi Uhre Nielsen et al.
PloS one, 15(9), e0238524-e0238524 (2020-09-12)
Markers of lung inflammation measured directly in expectorated sputum have the potential of improving the timing of antibiotic treatment in cystic fibrosis (CF). L-Lactate might be a marker of inflammation, as it is produced from glucose by polymorphonuclear neutrophils (PMNs)
Articles
Warburg effect enhances glucose to lactate conversion in tumor cells, regardless of oxygen levels; impacting cancer metabolism since 1924.
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