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MAK054

Sigma-Aldrich

α-Ketoglutarate Assay Kit

sufficient for 100 colorimetric or fluorometric tests

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 100 colorimetric or fluorometric tests

detection method

colorimetric
fluorometric

relevant disease(s)

cancer

storage temp.

−20°C

General description

α-Ketoglutarate (α-KG) is a key intermediate in the Tricarboxylic Acid (TCA) Cycle, formed by the oxidative decarboylation of isocitrate by isocitrate dehydrogenase. The synthesis of α-KG from glutamate by glutamate dehydrogenase is a key TCA cycle anaplerotic reaction. α-KG is an important nitrogen transporter. This α-Ketoglutarate Assay Kit provides a simple, sensitive and rapid means for quantifying α-KG in a variety of samples.

Application

α-Ketoglutarate Assay Kit has been used to determine the activity of α-ketoglutarate.[1][2][3]

Suitability

Suitable for the measurement of a-Ketoglutarate in a variety of biological samples including cell and tissue lysates

Principle

α-KG concentration is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/ fluorometric (λex = 535/λem = 587 nm) product, proportional to the α-KG present. Typical sensitivities of detection for this kit are 0.01 to 10 nmoles.

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Health hazard
GHS08

signalword

Danger

hcodes

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

10 - Combustible liquids

flash_point_f

188.6 °F - closed cup

flash_point_c

87 °C - closed cup

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Certificates of Analysis (COA)

Lot/Batch Number
8J28K06770
8B11K06770
7F14K06770
7A22K06770
6H12K06770

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Gunars Osis et al.
Physiological reports, 4(8) (2016-04-28)
The bicarbonate transporter, NBCe1 (SLC4A4), is necessary for at least two components of the proximal tubule contribution to acid-base homeostasis, filtered bicarbonate reabsorption, and ammonia metabolism. This study's purpose was to determine NBCe1's role in a third component of acid-base
Phoebe J Benson et al.
Frontiers in microbiology, 7, 475-475 (2016-04-20)
Interest in the production of carbon commodities from photosynthetically fixed CO2 has focused attention on cyanobacteria as a target for metabolic engineering and pathway investigation. We investigated the redirection of carbon flux in the model cyanobacterial species, Synechococcus elongatus PCC
Qun Zhou et al.
Bioscience reports, 39(4) (2019-03-25)
Long intergenic non-coding RNA p21 (lincRNA-p21) is down-regulated in some solid tumors. Glutamine catabolism plays an important role in cancer development. However, the role of lincRNA-p21 and its association with glutamine catabolism remain unknown in bladder cancer (BC). In the
Jianjian Zhao et al.
Bioscience reports, 40(3) (2020-03-04)
The activation of hepatic stellate cells (HSCs) plays a central role in liver fibrosis. α-ketoglutarate is a natural metabolite and previous studies have shown that increase in intracellular α-ketoglutarate can inhibit HSC activation. The aim of the present study is
Effect of NBC e1 deletion on renal citrate and 2-oxoglutarate handling.
Osis, et al.
Physiological Reports, 4(8), e12778-e12778 (2016)
View All Related Papers

Articles

Glutamine Metabolism in Cancer Cells

Sigma article discusses tumor cell metabolic pathways, focusing on aerobic glycolysis and mitochondrial activity.

Protocols

Chiral LC-MS/MS of D- and L-2-Hydroxyglutaric Acid Biomarkers

We describe here a rapid and sensitive method to separate and measure D-2-OHG and L-2-OHG enantiomers using high-resolution mass spectrometry (HRMS) detection.

Questions

1–4 of 4 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  3. Is a 10KDa MWCO spin filter a suitable substitute for perchloric acid in the deproteinization step of the Acetyl-CoA Assay Kit (MAK039)?

    1 answer

    1. Perchloric acid (PCA) is recommended for deproteinization, but if unavailable, 10 KDa spin filters can be used as an alternative.

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  4. この製品は植物（シロイヌナズナ）でも測定可能ですか。

    1 answer

    1. This kit would be suitable for plant samples assuming that the concentration of a-Ketoglutarate present in the tissue lysate is sufficient to enable detection. This α-Ketoglutarate Assay Kit is suitable for the measurement of a-Ketoglutarate in biological samples including cell and tissue lysates. The minimum amount of a-Ketoglutarate used to set up the standard curve is 2 nmole per well. See the link below to review the protocol:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/644/013/mak054bul.pdf

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