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MAK043

Sigma-Aldrich

Cholesterol Quantitation Kit

sufficient for 100 colorimetric or fluorometric tests

Synonym(s):

Cholesterol Assay Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.25

usage

sufficient for 100 colorimetric or fluorometric tests

detection method

colorimetric
fluorometric

relevant disease(s)

cancer; cardiovascular diseases

storage temp.

−20°C

General description

Cholesterol is an important component of mammalian cell membranes where it functions in intracellular transport, cell signaling, and maintaining membrane fluidity. Within the blood, cholesterol circulates as both the free acid and as cholesterol esters. Controlling serum cholesterol has an important therapeutic role as elevated cholesterol levels are associated with the development of atherosclerosis and cardiovascular pathologies. Recent evidence suggests a disturbance of cholesterol homeostasis contributes to the development of a chronic inflammatory state.

Cholesterol is predominantly produced in the liver.[1] Abnormal cholesterol levels are implicated in coronary heart disease.[2] Cholesterol is associated with breast cancer progression or inhibition. Tumor cells are capable of synthesizing their own cholesterol, which aids in cell proliferation.[1] Increased cholesterol level is observed in cancers, especially in prostate cancer.[3] Elevated serum cholesterol is observed in obesity.[2]

Application

Cholesterol Quantitation Kit has been used to measure the total serum cholesterol level.[4][5][6]

Suitability

Suitable for use with cell and tissue culture samples, urine, plasma, serum, and other biological samples.

Principle

The Cholesterol Quantitation Kit can also be used to determine the concentration of free cholesterol, cholesteryl esters, or both (total) present in a sample. In this kit, total cholesterol concentration is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/fluorometric (λex = 535/λem = 587 nm) product, proportional to the cholesterol present.

replaced by

Product No.
Description
Pricing

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

10 - Combustible liquids

flash_point_f

188.6 °F - closed cup

flash_point_c

87 °C - closed cup

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
8L11K06030
8A17K06030
8C01K06030
8G11K06030
7J02K06030

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Cholesterol and Cancer, in the Balance.
Sandrine S P and Poirot M
Science, 343(6178), 1445-1446 (2014)
Hypolipidemic and Anti-Atherogenic Activity of Aqueous Extract of Leaves of Lagenaria siceraria in Wistar Rats.
Aslam M and Nijam R
Journal of Natural Remedies, 14(1), 52-57 (2013)
Mingzhi Han et al.
EMBO molecular medicine, 12(1), e10924-e10924 (2019-11-30)
Dysregulated cholesterol metabolism is a hallmark of many cancers, including glioblastoma (GBM), but its role in disease progression is not well understood. Here, we identified cholesterol 24-hydroxylase (CYP46A1), a brain-specific enzyme responsible for the elimination of cholesterol through the conversion
Time trends in population cholesterol levels 1986--2004: influence of lipid-lowering drugs, obesity, smoking and educational level. The northern Sweden MONICA study
Eliasson M, et al.
J. Int. Med., 260(6), 551-559 (2006)
Alessandro Attanzio et al.
Journal of the science of food and agriculture, 99(9), 4242-4247 (2019-02-26)
The stems of Opuntia ficus-indica, known as cladodes, are a rich source of soluble fibers, which makes them an important candidate for the production of functional foods. Tagliatelle of durum wheat fortified with Opuntia cladode extract (OCE) at different levels
View All Related Papers

Questions

1–10 of 10 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  3. Hi, Can the cholesterol quantification assay (MAK043) measure the cholesterol content in lipid nanoparticles? Does it break down the particles and measure total cholesterol or does it measure only unreacted cholesterol?

    1 answer

    1. The Cholesterol Quantitation Kit can also determine the concentration of free cholesterol, cholesteryl esters, or both (total) present in a sample. The assay is enzyme-based and needs the cholesterol or cholesterol esters in solution rather than in a membrane type structure. The kit has not been tested directly for use with lipid nanoparticles.

      Helpful?

  4. Can you use this kit on frozen animal cell pellets?

    1 answer

    1. It is recommended to use fresh samples for this kit. However, although not ideal, flash-frozen tissue or cells stored at -80C can be used. It is important to remember that results may vary between fresh and frozen samples. If possible, fresh and frozen samples should be run parallel to compare results.

      Helpful?

  5. Can this kit be used with bacterial supernatant (cultivation medium without cells) and how prepare these samples? Is necessary organic extraction?

    1 answer

    1. This product has not been tested for use in bacterial culture supernatent, however there is no reason to suspect that this kit would not be suitable. Solid tissues and cell lysates are extracted using chloroform:isopropanol:Igepal preparation. Samples such as serum, plasma, or other biological fluids do not require organic extraction. The minimum concentration for detection has not been determined, although the first data point in the standard curve is 1 ug/well for the colorimetric assay and 0.1 ug/well for the fluorometric assay. See the link below to review the product datasheet which includes the protocol.
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/420/556/mak043bul.pdf

      Helpful?

  6. What's the limit of detection (LOD) and detection range for cholesterol quantitation kit?

    1 answer

    1. The absolute limit of detection of this kit is not determined. However the kit is able to quantify at least 1 ug/well (colorimetric) or 0.1 ug/well (fluorimetric).

      Helpful?

  7. Hi, we need to quantify cholesterol in shrimps mescle samples, are possible?

    1 answer

    1. Product MAK043 is suitable for detection of cholesterol in tissues and cell samples as well as urine, plasma, serum, or other biological materials. See the link below to review to protocol which includes sample preparation for tissues.
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/420/556/mak043bul.pdf

      Helpful?

  8. We want to quantify the cholesterol of mice feces. Can we use this kit for mice fecal?

    1 answer

    1. Yes, this kit is suitable for use with cell and tissue culture samples, urine, plasma, serum, and other biological samples including feces.

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  9. Can these kits be used with serum samples from mouse?

    1 answer

    1. Yes, this kit can be used on mouse serum samples. We generally recommend that fresh serum samples be used. Frozen samples can probably be used. However, the successful use of frozen samples depends on the efficiency of freezing them and the amount of time they have been frozen. If previously frozen serum samples are used, you may want to check the performance of the kit with fresh vs. frozen samples.

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  10. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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