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MAK038

Sigma-Aldrich

NADP/NADPH Quantitation Kit

sufficient for 100 colorimetric tests

Synonym(s):

NADP/NADPH Assay Kit

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About This Item

UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.25

usage

sufficient for 100 colorimetric tests

detection method

colorimetric

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−70°C

General description

Nicotinamide adenine dinucleotide phosphate (NADP) is an enzymatic cofactor involved in many redox reactions where it cycles between the reduced (NADPH) and oxidized (NADP) forms.[1] NADP is also involved in biosynthetic reactions such as lipid and nucleic acid synthesis where it functions as a reducing agent.[2] The oxidative branch of the Pentose Phosphate Pathway (PPP) is the major source of NADPH produced in animal cells.[3]

Application

NADP/NADPH (nicotinamide adenine dinucleotide phosphate) Quantitation Kit has been used for quantification of NADP/NADPH.[4][5][6] It has been used to quantify NAD(P)H in order to determine SHMT2 (serine hydroxymethyltransferase 2) specific enzyme activity.[7]

Suitability

Suitable for the detection of NADP, NADPH and their ratio in a variety of biological samples including cell and tissue lysates

Principle

The NADP/NADPH Quantification Kit provides a convenient tool for sensitive detection of the intracellular nucleotides, NADP (nicotinamide adenine dinucleotide phosphate) and NADPH, and their ratio. There is no requirement to purify NADP/NADPH from samples. This assay is specific for NADP and NADPH and does not detect NAD or NADH. NADPt (NADP and NADPH) or NADPH are quantified in a colorimetric assay (450 nm).

replaced by

Product No.
Description
Pricing

pictograms

Health hazardCorrosion
GHS08,GHS05

signalword

Danger

Hazard Classifications

Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1

Storage Class

8A - Combustible corrosive hazardous materials

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
8L23K03470
7K29K03470
8E25K03470
7E20K03470
7D16K03470

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Luigi Mele et al.
Cell death & disease, 9(5), 572-572 (2018-05-16)
Pentose phosphate pathway (PPP) is a major glucose metabolism pathway, which has a fundamental role in cancer growth and metastasis. Even though PPP blockade has been pointed out as a very promising strategy against cancer, effective anti-PPP agents are not
Q Shi et al.
Toxicology, 381, 64-74 (2017-02-28)
It has been well established that inflammation and concurrent mutagenic exposures drive the carcinogenic process in a synergistic way. To elucidate the role of the inflammatory cytokine IL-8 in this process, we studied its effect on the activation and deactivation
Andreas Carstensen et al.
Plant physiology, 177(1), 271-284 (2018-03-16)
Phosphorus (P) is an essential macronutrient, and P deficiency limits plant productivity. Recent work showed that P deficiency affects electron transport to photosystem I (PSI), but the underlying mechanisms are unknown. Here, we present a comprehensive biological model describing how
Metabolic engineering of Escherichia coli: increase of NADH availability by overexpressing an NAD+-dependent formate dehydrogenase.
Berri'os-Rivera S J, et al.
Metabolic engineering, 4(3), 217-229 (2002)
Mechanisms of action of isoniazid.
Timmins G S and Vojo D
Molecular Microbiology, 62(5), 1220-1227 (2006)
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Questions

1–3 of 3 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  3. Is it possible to have product information sheet to know more about the details of this product please?

    1 answer

    1. Please see the link below to review the product datasheet, including the protocol, for this kit:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/200/280/mak038bul.pdf

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