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MAK002

Sigma-Aldrich

L-Amino Acid Quantitation Kit

sufficient for 100 colorimetric or fluorometric tests

Synonym(s):

L-Amino Acid Assay Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.25

usage

sufficient for 100 colorimetric or fluorometric tests

detection method

colorimetric
fluorometric

storage temp.

−20°C

General description

The new L-Amino Acid Assay Kit, MAK490, is now available!

L-amino acids are essential molecules defined by the nature of their side-chain. There are 20 amino acids that are commonly used by most organisms for the production of proteins and other biologically relevant molecules.[1] While amino acids can occur in either the D- or L-enantimer, only the L-form is used by cells.[2]

Suitability

Suitable for quantitating L-amino acids in body fluids or purified samples.

Principle

In this assay, L-Amino Acid concentration is determined by a coupled enzyme reaction, which results in a colorimetric (570 nm)/fluorometric (λex = 535/λem = 587 nm) product, proportional to the L-Amino Acids present. Glycine is not detectable by this assay.

replaced by

Product No.
Description
Pricing

pictograms

Skull and crossbonesHealth hazard
GHS06,GHS08

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Danger

Hazard Classifications

Acute Tox. 1 Inhalation - Acute Tox. 2 Dermal - Acute Tox. 2 Oral - Aquatic Chronic 3 - Resp. Sens. 1 - Skin Sens. 1

Storage Class

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

flash_point_f

188.6 °F

flash_point_c

87 °C

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
8J19K06390
8G20K06390
8B15K06390
7L09K06390
6K29K06390

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Analysis of endogenous D-amino acid-containing peptides in metazoa.
Bai L, et al.
Bioanalytical reviews., 1(1), 7-24 (2009)
Alberts B, et al.
Molecular Biology in Plant Pathogenesis and Disease Management (2002)
Jinrang Kim et al.
Endocrinology, 160(5), 979-988 (2019-04-03)
Plasma amino acids and their transporters constitute an important part of the feedback loop between the liver and pancreatic α-cell function, and glucagon regulates hepatic amino acid turnover. Disruption of hepatic glucagon receptor action activates the loop and results in
Alberts B, et al.
Molecular biology of the Cell. 4th Edition. (2002)
Peng Shang et al.
Aging cell, 16(2), 349-359 (2017-01-14)
The dry (nonneovascular) form of age-related macular degeneration (AMD), a leading cause of blindness in the elderly, has few, if any, treatment options at present. It is characterized by early accumulation of cellular waste products in the retinal pigmented epithelium
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Questions

1–3 of 3 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. Could you please provide me with the detection sensitivity limit of the above product ?

    1 answer

    1. The absolute lowest limit of detection for this method has not been determined. The kit has been optimized to detect at least 8 nmole per well based on the standard curve set up. See the link below to review the product datasheet which includes the protocol:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/126/216/mak002bul.pdf

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