immunohistochemistry (frozen sections): 1-2 μg/mL using rat skeletal muscle and rat kidney western blot: 1-2 μg/mL using cytosolic fraction of rat skeletal muscle
Myopodin (80-95 kDa), a novel actin bundling protein, is an additional member of the synaptopodin gene family. Myopodin is expressed in skeletal and cardiac muscles. Myopodin contains one PPXY motif, multiple PXXP motifs, and a nuclear export sequence (NES). In the-disc, myopodin colocalizes with α-actinin. Myopodin, like several actin-bundling proteins, has been shown to shuttle between the nucleus and cytoplasm. It is localized in nucleus in myoblasts.
Immunogen
synthetic peptide encoding amino acids 566-585 located at the mid-region of human myopodin, conjugated to KLH. This sequence is highly conserved (77% sequence identity) in mouse myopodin and is not found in human or rat synaptopodin.
Application
Anti-Myopodin has been used in:
immunohistochemistry
immunostaining
western blot analysis
Biochem/physiol Actions
Myopodin directly binds to actin and contains an actin-binding site in the centre of the protein. Myopodin has actin bundling activity as shown by lantraculin - a sensitive cytosolic actin bundles and nuclear actin loops in transfected cells expressing GFP-myopodin. It binds to stress fibres in a punctuated pattern into the Z-disc during myotube differentiation. Myopodin is frequently down-regulated in invasive stages of some types of cancers, including invasive bladder tumors. Frequent complete or partial deletions of the myopodin gene have been shown to occur in 80% of invasive prostate cancer cases. Expression of myopodin induces suppression of tumor growth both in vivo and in vitro. Myopodin inhibits tumor metastasis by functioning as a tumor suppressor gene.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Myopodin, a synaptopodin homologue, is frequently deleted in invasive prostate cancers
Lin F, et al.
The American Journal of Pathology, 159(5), 1603-1612 (2001)
Interaction between importin 13 and myopodin suggests a nuclear import pathway for myopodin
Liang J, et al.
Molecular and Cellular Biochemistry, 307(1-2), 93-100 (2008)
Expression of myopodin induces suppression of tumor growth and metastasis
Jing L, et al.
The American Journal of Pathology, 164(5), 1799-1806 (2004)
Tumor suppressor role for myopodin in bladder cancer: loss of nuclear expression of myopodin is cell-cycle dependent and predicts clinical outcome
Sanchez-Carbayo M, et al.
Oncogene, 22(34), 5298-5298 (2003)
Differentiation-and stress-dependent nuclear cytoplasmic redistribution of myopodin, a novel actin-bundling protein
Weins A, et al.
The Journal of Cell Biology, 155(3), 393-404 (2001)
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