M9426

MAP Kinase, N-terminal histidine-tagged non-activated protein from rat

≥95% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, 500 U/mg (Can be activated by MEK to more than 500 units per mg)

• Synonym(s): ERK2, Extracellular-signal regulated kinase, MAPK, Mitogen activated protein kinase
• CAS Number: 137632-08-7
• MDL number: MFCD00677284
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• biological source: rat
• Quality Level: 200
• recombinant: expressed in E. coli
• assay: ≥95% (SDS-PAGE)
• form: lyophilized powder
• specific activity: 500 U/mg (Can be activated by MEK to more than 500 units per mg)
• mol wt: 42 kDa
• UniProt accession no.: P63086
• application(s): cell analysis
• storage temp.: −20°C
• Gene Information: rat ... Mapk1(116590)

Description

Biochem/physiol Actions

MAP kinase is a serine/threonine kinase that is activated by MEK through a phosphorylation cascade. The cascade starts with extracellular signals transmitted by growth factor-regulated receptor tyrosine kinases or G-protein coupled receptors. Dually phosphorylated (Thr and Tyr) MAP kinase translocates from the cytoplasm to the nucleus, where it phosphorylates protein transcription factors, transducing extracellular signals into genomic responses, proliferation or differentiation.

Unit Definition

One unit of activated MAP kinase will transfer 1 nmol of phosphate from ATP to myelin basic protein per min at 30 °C.

Physical form

Lyophilized powder containing Tris, pH 7.5, NaCl, and EGTA, with DTT and trehalose as stabilizers

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)