M9192
Anti-MRP1 antibody ,Mouse monoclonal
clone QCRL-4, purified from hybridoma cell culture
Synonym(s):
Anti-Multidrug Resistance Associated Protein 1
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About This Item
biological source
mouse
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
QCRL-4, monoclonal
form
buffered aqueous solution
mol wt
antigen ~190 kDa
species reactivity
human
concentration
~2 mg/mL
technique(s)
flow cytometry: 0.5-2 μg/mL using human H69AR cells
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
microarray: suitable
western blot: suitable
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... ABCC1(4363)
General description
Monoclonal Anti-MRP1 (mouse IgG1 isotype) is derived from the QCRL-4 hybridoma produced by the fusion of mouse myeloma cells (SP2/O) and splenocytes from mice. MRP1 protein belongs to the multidrug resistance-associated protein (MRP) subfamily of adenosine triphosphate (ATP)-binding cassette (ABC) transporters.
Multidrug resistance associated protein 1 (MRP1) is present in subcellular vesicles and is composed of 1531 amino acids. It has a molecular weight of 190kDa. The gene encoding MRP1 is localized in human chromosome 16p13.11.
Specificity
Does not cross-react with the human MDR1 and MDR3 proteins.
Immunogen
non-denatured membrane preparations of H69AR human small cell lung cancer cell line, which highly expresses MRP1. The epitope resides within the second nucleotide-binding domain of human MRP1 between amino acids 1294-1531.
Application
Anti-MRP1 antibody, Mouse monoclonal may be used in:
- noncompetitive capillary electrophoresis immunoassay (CEIA)
- immunoblotting
- flow cytometry
- immunoprecipitation
- transport inhibition
Biochem/physiol Actions
Multidrug resistance associated protein 1 (MRP1) functions in the metabolism of many drugs. It is involved in transporting different biomolecules and anticancer drugs like prostaglandins, glutathione, alkaloids and anthracyclines. MRP1 offers resistance against chemotherapy to cells in solid tumors.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
Storage and Stability
For continuous use, store at 2-8 °C for up to one month. For prolonged storage, freeze in working aliquots at −20 °C. Repeated freezing and thawing is not recommended. Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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E M Leslie et al.
Toxicology, 167(1), 3-23 (2001-09-15)
The 190 kDa multidrug resistance protein 1 (MRP1/ABCC1) is a founding member of a subfamily of the ATP binding cassette (ABC) superfamily of transport proteins and was originally identified on the basis of its elevated expression in multidrug resistant lung
Julius Mbuna et al.
Journal of chromatography. A, 1218(25), 3923-3927 (2011-05-17)
The adenosine triphosphate (ATP) binding-cassette (ABC) transporters are a superfamily of cellular proteins that have been partly implicated as a cause of multidrug resistance (MDR) in cancer cells. The ABC superfamily consists of P-glycoprotein, multidrug resistance-associated proteins (MRP) and breast
Shu-Li Shao et al.
Asian Pacific journal of cancer prevention : APJCP, 15(24), 10597-10601 (2015-01-22)
Up-regulation of multidrug resistance-associated protein 1 (MRP1) is regarded as one of the main causes for multidrug resistance (MDR) of tumor cells, leading to failure of chemotherapy-based treatment for a multitude of cancers. However, whether silencing the overexpressed MRP1 is
Yifan Hong et al.
Proceedings of the National Academy of Sciences of the United States of America, 112(11), 3211-3216 (2015-03-05)
Glutathione and multidrug resistance protein (MRP) play an important role on the metabolism of a variety of drugs. Bismuth drugs have been used to treat gastrointestinal disorder and Helicobacter pylori infection for decades without exerting acute toxicity. They were found
Tereza Kunická et al.
PloS one, 9(7), e101740-e101740 (2014-08-01)
ATP-Binding Cassette (ABC) transporters may cause treatment failure by transporting of anticancer drugs outside of the tumor cells. Multidrug resistance-associated protein 1 coded by the ABCC1 gene has recently been suggested as a potential prognostic marker in breast cancer patients.
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