M8433

Anti-phospho-c-Myc (pThr⁵⁸/pSer⁶²) antibody produced in rabbit

affinity isolated antibody, liquid

• MDL number: MFCD02097158
• UNSPSC Code: 12352203

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: liquid
• mol wt: antigen ~64-67 kDa
• species reactivity: rat, human, mouse
• technique(s): immunohistochemistry: 10-20 μg/mL; immunoprecipitation (IP): 2-4 μg/mL; western blot: 0.5-4 μg/mL
• UniProt accession no.: P01106
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: phosphorylation (pThr58/pSer62)
• Gene Information: human ... MYC(4609) ; mouse ... Myc(17869) ; rat ... Myc(24577)

Description

General description

The transcription factor c-Myc is a short-lived nuclear phosphoprotein involved in cell proliferation, differentiation, and cancer. Expression of c-Myc is strictly controlled by mitogens and is required for cell cycle entry. c-Myc binds to DNA and activates transcription as a heterodimeric complex with Max. c-Myc is phosphorylated in vitro at Ser⁶² by MAP Kinase, and in vivo at both Thr⁵⁸ and Ser⁶² although the pathways and kinases acting in vivo have not been identified. Mutation of Thr⁵⁸ and Ser⁶² to Ala inhibits the ability of c-Myc to stimulate transcription. c-Myc, a ~45 kDa protein that migrates with an apparent molecular weight of ~64-67kDa in reducing SDS-PAGE, appears to regulate multiple aspects of growth control; c-Myc promotes cell proliferation in the presence of Cdk inhibitors and induces apoptosis upon overexpression in serum deprived cells.

Specificity

Does not react with non-phosphorylated c-Myc.

Immunogen

synthetic peptide corresponding to residues around phospho-Thr⁵⁸ and phospho-Ser⁶² of human c-Myc.

Physical form

In phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% thimerosal.

Preparation Note

Purified by protein A and peptide affinity chromatography.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable