M8069
Monoclonal Anti-Mad1 antibody produced in mouse
~2 mg/mL, clone 9B10, purified immunoglobulin, buffered aqueous solution
Synonym(s):
Anti-Mitotic Arrest Deficient
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About This Item
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biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
9B10, monoclonal
form
buffered aqueous solution
mol wt
antigen ~85 kDa
species reactivity
human
concentration
~2 mg/mL
technique(s)
immunocytochemistry: 10-20 μg/mL using HeLa cells
immunoprecipitation (IP): suitable
microarray: suitable
isotype
IgG2b
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... MAD1L1(8379)
General description
Mitotic arrest deficient-like 1 (MAD1) is part of the cell-cycle checkpoint.
Monoclonal Anti-Mad1 (mouse IgG2b isotype) is derived from the hybridoma 9B10 produced by the fusion of mouse myeloma cells (SP2/0 cells) and splenocytes from BALB/c mice immunized with human recombinant Mad1 protein.
Immunogen
recombinant human Mad1 protein.
Application
Monoclonal Anti-Mad1 antibody produced in mouse has been used in
- immunoblotting
- immunoprecipitation
- immunocytochemistry
Biochem/physiol Actions
Mitotic arrest deficient-like 1 (MAD1) functions as a regulator in the segregation of chromosomes during mitosis. It recruits mitotic arrest deficient-like 2 (MAD2) to kinetochores and directs it to function as an inhibitor. MAD1 also helps MAD2 to associate with translocated promoter region (TPr).
Physical form
Solution in 0.01 M phosphae buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Jun Wan et al.
Current biology : CB, 24(22), 2687-2692 (2014-12-03)
Mitotic arrest deficient 1 (Mad1) plays a well-characterized role in the major cell-cycle checkpoint that regulates chromosome segregation during mitosis, the mitotic checkpoint (also known as the spindle assembly checkpoint). During mitosis, Mad1 recruits Mad2 to unattached kinetochores, where Mad2
Garry G Sedgwick et al.
mAbs, 8(4), 689-697 (2016-03-18)
The spindle assembly checkpoint (SAC) ensures accurate chromosome segregation during mitosis by delaying the activation of the anaphase-promoting complex/cyclosome (APC/C) in response to unattached kinetochores. The Mad2 protein is essential for a functional checkpoint because it binds directly to Cdc20
Timo E F Kuijt et al.
Chromosoma, 123(5), 471-480 (2014-04-04)
Fidelity of chromosome segregation is monitored by the spindle assembly checkpoint (SAC). Key components of the SAC include MAD1, MAD2, BUB1, BUB3, BUBR1, and MPS1. These proteins accumulate on kinetochores in early prometaphase but are displaced when chromosomes attach to
Efficient mitotic checkpoint signaling depends on integrated activities of Bub1 and the RZZ complex.
Gang Zhang et al.
The EMBO journal, 38(7) (2019-02-21)
Kinetochore localized Mad1 is essential for generating a "wait anaphase" signal during mitosis, hereby ensuring accurate chromosome segregation. Inconsistent models for the function and quantitative contribution of the two mammalian Mad1 kinetochore receptors: Bub1 and the Rod-Zw10-Zwilch (RZZ) complex exist.
Yuqing Zhang et al.
Communications biology, 7(1), 164-164 (2024-02-10)
Accurate mitosis is coordinated by the spindle assembly checkpoint (SAC) through the mitotic checkpoint complex (MCC), which inhibits the anaphase-promoting complex or cyclosome (APC/C). As an essential regulator, Cdc20 promotes mitotic exit through activating APC/C and monitors kinetochore-microtubule attachment through
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