M6569

Anti-MBD1 antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

• MDL number: MFCD06411841
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~80 kDa
• species reactivity: human
• concentration: ~1 mg/mL
• technique(s): indirect immunofluorescence: 1-2 μg/mL using HeK 293T cells transfected with MBD1 (isoform 3), fixed with paraformaldehyde-triton; microarray: suitable; western blot: 0.25-0.5 μg/mL using extracts of HEK 293T cells transfected with MBD1 (isoform 3)
• UniProt accession no.: Q9UIS9
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... MBD1(4152)

Description

General description

MBD1 gene is mapped to human chromosome 18q21.1.

Methyl-CpG binding domain protein 1 (MBD1) exists as five isoforms.

Specificity

Anti MBD1 recognizes recombinant human MBD1 (~80 kDa).

Immunogen

synthetic peptide corresponding to amino acids 591-605 of human MBD1, conjugated to KLH via an N-terminal added lysine residue. This sequence is found in MBD1 isoforms.

Application

Anti-MBD1 antibody produced in rabbit may be used for immunofluorescence and immunoblotting.

Biochem/physiol Actions

MBD1 can act as a transcriptional repressor. It mediates transcriptional repression by recruiting heterochromatin-binding protein (HP1), the histone methyl transferase Suv39h1 and histone deacetylase using the methyl binding domain (MBD). The transcriptional repression domain (TRD) of MBD1 mediates its interaction with MBD1-containing chromatin-associated factor (MCAF-1).

Methyl-CpG binding domain protein 1 (MBD1) aids pancreatic cancer cells in drug resistance and contributes to disease progression. It gets recruited to those sites where DNA damage has occurred and the protein has also shown to be involved in suppression of gene transcription. MBD1 acts as a link between methylation of DNA and repression of transcription.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilu-tions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.