M6167
Macrophage Inflammatory Protein-1α from mouse
≥97% (SDS-PAGE and N-terminal analysis), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
Synonym(s):
MIP-1α
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About This Item
MDL number:
UNSPSC Code:
51111800
biological source
mouse
recombinant
expressed in E. coli
assay
≥97% (SDS-PAGE and N-terminal analysis)
form
lyophilized powder
potency
0.406 ng/mL EC50
packaging
pkg of 10 μg
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
UniProt accession no.
storage temp.
−20°C
Gene Information
mouse ... Ccl3(20302)
Physical form
Lyophilized from a 0.2 μm filtered solution in 30% acetonitrile and 0.1% trifluoroacetic acid containing 0.5 mg bovine serum albumin
Analysis Note
The biological activity is measured by its ability to chemoattract cultured human monocytes or mouse BaF/3 cells transfected with human CCR5.
Storage Class
13 - Non Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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G J Graham et al.
Nature, 344(6265), 442-444 (1990-03-29)
The haemopoietic system has three main compartments: multi-potential stem cells, intermediate stage progenitor cells and mature cells. The availability of simple reproducible culture systems has made possible the characterization and purification of regulators of the progenitor cells, including colony-stimulating factors
Grace F T Poon et al.
Journal of immunology (Baltimore, Md. : 1950), 195(2), 632-642 (2015-06-19)
Although classical dendritic cells (DCs) arise from distinct progenitors in the bone marrow, the origin of inflammatory DCs and the distinction between monocyte-derived DCs and macrophages is less clear. In vitro culture of mouse bone marrow cells with GM-CSF is
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