M5698
Anti-MIB2 (C-terminal) antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution
Synonym(s):
Anti-Mindbomb homolog 2, Anti-Novel zinc finger protein, Anti-Novelzin, Anti-Skeletrophin, Anti-Zinc finger ZZ type with ankyrin repeat domain protein 1
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
biological source
rabbit
conjugate
unconjugated
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~110 kDa
species reactivity
human, rat
technique(s)
western blot: 1:500-1:1,000 using lysates of rat skeletal muscle
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... MIB2(142678)
General description
Mindbomb homolog 2 (MIB2) which is also known as skeletrophin, is an ubiquitin ligase. The gene encoding it is localized on human chromosome 1p36.32.
Mouse MIB1 and MIB2 share 52% similarity in their amino acid sequences and contain a similar domain organization.These include two homologous to the E6-AP carboxyl terminus (HECT) domains. It also comprises RCC1-like domain (RLD) domain containing E3 ubiquitin protein ligase 2 (herc2)/mib domains.Other domains associated with MIB2 are one ZZ zinc finger, one mib repeat, ankyrin repeats, and two interesting new gene (RING) domains. MIB2 is highly expressed in adult tissues.
Specificity
Anti-MIB2 (C-terminal) specifically recognizes human and rat MIB2.
Immunogen
synthetic peptide corresponding to amino acides 950-966 of human MIB2 conjugated to KLH. The corresponding sequence differs by one amino acid in mouse.
Application
Anti-MIB2 (C-terminal) antibody produced in rabbit may be used in immunoblotting.
Biochem/physiol Actions
Disruption of the MIB2 gene results in exencephaly and appears to be less essential in mouse development.
Mindbomb homolog 2 (MIB2) takes part in the ubiquitination of Notch ligands like Jagged-2 (JAG2). It binds to actin and CYLD (cylindromatosis (turban tumor syndrome)), which is an ubiquitin hydrolase. It has also been shown that MIB2 negatively regulates the transcription of Met oncogene.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Certificates of Analysis (COA)
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Katrin Domsch et al.
PloS one, 12(3), e0173733-e0173733 (2017-03-11)
The proper differentiation and maintenance of myofibers is fundamental to a functional musculature. Disruption of numerous mostly structural factors leads to perturbations of these processes. Among the limited number of known regulatory factors for these processes is Mind bomb2 (Mib2)
Bon-Kyoung Koo et al.
PloS one, 2(11), e1221-e1221 (2007-11-29)
The Notch signaling pathway is an evolutionarily conserved intercellular signaling module essential for cell fate specification that requires endocytosis of Notch ligands. Structurally distinct E3 ubiquitin ligases, Neuralized (Neur) and Mind bomb (Mib), cooperatively regulate the endocytosis of Notch ligands
Jiang I Wu et al.
Genesis (New York, N.Y. : 2000), 45(11), 722-727 (2007-11-08)
Mib1 and Mib2 ubiquitin ligases are very similar in their domain construction. They partake in the Notch signaling pathway by ubiquitinating the Notch receptors Delta and Jagged prior to endocytosis. We have created a targeted mutation of Mib2 and show
T Takeuchi et al.
Oncogene, 25(53), 7059-7069 (2006-05-23)
Skeletrophin (mindbomb homolog 2 (MIB2)) is a RING (Really Interesting New Gene) finger-dependent ubiquitin ligase, which targets the intracellular region of Notch ligands. A previous immunohistochemical study demonstrated that skeletrophin was downregulated in many melanomas. In the present study, we
Tamotsu Takeuchi et al.
DNA and cell biology, 24(5), 339-344 (2005-05-05)
Recent experiments have revealed that aberrant functionality of the chromatin remodeling complex is related to tumorigenicity in various malignant tumors. Skeletrophin is an actin-binding cytoskeleton-related molecule, which is induced by the overexpression of truncated human SWI1 (SMARCF1). Human SWI1 is
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