M4308
Monoclonal Anti-MDM2 antibody produced in mouse
clone SMP14, ascites fluid
Synonym(s):
Anti-ACTFS, Anti-HDMX, Anti-LSKB, Anti-hdm2
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About This Item
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biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
SMP14, monoclonal
mol wt
antigen ~90 kDa (additional band of approx. 55 kDa)
contains
15 mM sodium azide
species reactivity
human, rat, mouse (weak)
technique(s)
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunohistochemistry (frozen sections): suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 1:1,000 using a whole extract of transfected 293T (human embryonal kidney) cells expressing human MDM2
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... MDM2(4193)
mouse ... Mdm2(17246)
rat ... Mdm2(246362)
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General description
Monoclonal Anti-MDM2 (mouse IgG1 isotype) is derived from the SMP14 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse. The murine double minute (mdm2), an E3 ubiquitin ligase comprises an N-terminal p53 domain. The C-terminal domain encompasses the really interesting new gene (RING) domain, nuclear localization signal (NLS), nucleolar localization signal (NoLS) and a nuclear export signal (NES). MDM2 is mapped to the human chromosome 12q15. It is expressed as different splice variants.
Specificity
Detects the MDM2 protein alone or in complex with E2F1, p53, and retinoblastoma proteins. A slight cross-reactivity is observed with cytokeratins 6, 14, and 16.
Immunogen
synthetic peptide corresponding to amino acids 154-167 of human MDM2 conjugated to KLH.
Application
Monoclonal Anti-MDM2 antibody produced in mouse has been used in:
- immunoblotting
- immunoprecipitation (1 μg/ml)
- fluorescence-activated cell sorting (FACS)
- immunofluorescence (1:200)
- immunohistochemistry
- confocal immunofluorescence
- immunocytochemistry
- enzyme-linked immunosorbent assay (ELISA)
Biochem/physiol Actions
Murine double minute (mdm2) mediates the transactivation of the tumor suppressor p53. MDM2 binds to the histone acetyltransferase p300 transcriptional coactivator/histone acetylase to mediate the degradation of p53. The nuclear localization signal of MDM2 mediates its shuttling from the nucleus to the cytoplasm and mediates p53 degradation. In addition to its relationships with p53, MDM2 activates cell proliferation by stimulating the S-phase-inducing transcription factors, E2F1/dimerization partner 1 (DP1). The amplification of the MDM2 gene is detected in the esophagogastric, liver, colon, breast and lung carcinomas. A disturbance in the optimal levels of the MDM2/p53 axis may have an impact on premature aging. Monoclonal antibody reacting specifically with MDM2 is an essential tool in defining the interactions and distributions of MDM2, its function in signaling pathways and mutual regulation of p53.
Physical form
Monoclonal Anti-MDM2 is supplied as ascites fluid containing 15 mM sodium azide.
Storage and Stability
For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Description
Pricing
Storage Class
10 - Combustible liquids
wgk_germany
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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