M3068
Monoclonal Anti-MGMT antibody produced in mouse
clone MGMT-214, purified immunoglobulin, buffered aqueous solution
Synonym(s):
Anti-O6-Methylguanine-DNA Methyltransferase
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
Recommended Products
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
MGMT-214, monoclonal
form
buffered aqueous solution
mol wt
antigen ~25 kDa
species reactivity
human
technique(s)
indirect ELISA: suitable
microarray: suitable
western blot: 2-4 μg/mL using total cell extracts of HeLa cells
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... MGMT(4255)
General description
Monoclonal Anti-MGMT (O6-Methylguanine-DNA Methyltransferase) (mouse IgG1 isotype) is derived from the MGMT-214 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. O6-Methylguanine-DNA Methyltransferase (MGMT) is a DNA repair protein. MGMT gene is mapped to human chromosome 10q26.3. It comprises a C-terminal helix-turn-helix motif and arginine “finger,” flip in its active site. MGMT is expressed ubiquitously.
Specificity
Monoclonal Anti-MGMT (O6-Methylguanine-DNA Methyltransferase) recognizes human MGMT(approx. 25 kDa).
Immunogen
recombinant human MGMT.
Application
Monoclonal Anti-MGMT antibody produced in mouse may be used in enzyme-linked immunosorbent assay (ELISA) and immunoblotting.
Biochem/physiol Actions
O6-Methylguanine-DNA Methyltransferase (MGMT) removes O6-alkyl-guanine, primarily O6-methylguanine lesions from damaged DNA. It is a major contributor to cellular protection from the mutagenic, carcinogenic, and cytotoxic effects of DNA alkylation. The mechanism of MGMT action is based on the transfer of the alkyl group from the DNA to a unique acceptor cysteine residue in the protein. MGMT is frequently classified as a suicide protein. Because the reaction irreversibly inactivates the enzyme, the repair capacity for O6-methylguanine is dependent on the number of MGMT molecules in the cell. There is a correlation between the occurrence of cancer in various tissues and the lack of the MGMT enzyme. High levels of MGMT result in reduced tumor events and resistance of tumors to alkylating agents.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Product Selector Tool.
recommended
Product No.
Description
Pricing
Storage Class
10 - Combustible liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Choose from one of the most recent versions:
Certificates of Analysis (COA)
Lot/Batch Number
Don't see the Right Version?
If you require a particular version, you can look up a specific certificate by the Lot or Batch number.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Wei Yu et al.
Frontiers in oncology, 9, 1547-1547 (2020-02-06)
Chemoresistance has been a significant problem affecting the efficacy of drugs targeting tumors for decades. MGMT, known as O6-methylguanine-DNA methyltransferase, is a DNA repair enzyme that plays an important role in chemoresistance to alkylating agents. Hence, MGMT is considered a
Ricardo Gredilla et al.
Experimental gerontology, 45(7-8), 478-488 (2010-01-26)
Mitochondrial DNA is constantly exposed to oxidative injury. Due to its location close to the main site of reactive oxygen species, the inner mitochondrial membrane, mtDNA is more susceptible than nuclear DNA to oxidative damage. The accumulation of DNA damage
Dragony Fu et al.
Nature reviews. Cancer, 12(2), 104-120 (2012-01-13)
Alkylating agents constitute a major class of frontline chemotherapeutic drugs that inflict cytotoxic DNA damage as their main mode of action, in addition to collateral mutagenic damage. Numerous cellular pathways, including direct DNA damage reversal, base excision repair (BER) and
Z Q Zhou et al.
Proceedings of the National Academy of Sciences of the United States of America, 98(22), 12566-12571 (2001-10-19)
O(6)-methylguanine (O(6)mG) is a potent mutagenic and procarcinogenic DNA lesion. Organisms have evolved with a DNA repair mechanism that largely ameliorates the deleterious effects of O(6)mG through a direct reversal mechanism by a protein termed O(6)-methylguanine-DNA methyltransferase (MGMT). However, the
Hung-Sheng Shang et al.
Oncology reports, 36(4), 2207-2215 (2016-08-09)
Cervical cancer is one of the most common cancers in women worldwide and it is a prominent cause of cancer mortality. Curcumin is one of the major compounds from Turmeric and has been shown to induce cytotoxic cell death in
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service
