M249
Macrophage Inflammatory Protein-3α human
lyophilized powder (from PBS solution), suitable for cell culture
Synonym(s):
hMIP-3α, Exodus, LARC, MIP-3α
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About This Item
biological source
human
Quality Level
recombinant
expressed in E. coli
assay
≥95% (SDS-PAGE)
form
lyophilized powder (from PBS solution)
potency
0.3 nM ED50 (by calcium flux induction)
mol wt
~8 kDa
packaging
pkg of 25 μg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin tested
UniProt accession no.
storage temp.
−20°C
Gene Information
human ... CCL20(6364) , CCL20(6364)
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General description
MIP-3 α, also known as LARC or Exodus, is one of the many chemokines identified through the Expressed Sequence Tag database. MIP-3 alpha, cDNA encodes a 96 amino acid precursor protein and a 26 amino acid signal peptide. The resulting 70 amino acid mature protein has only 20-28% homology with other related human chemokines. The gene for MIP-3 alpha has been mapped to chromosome 2 rather than chromosome 17 where many human chemokine genes are located. Expression of the chemokine is detected in the lymphoid tissues, fetal lung and fetal liver. Recombinant and native MIP-3 alpha are known chemoattractants of lymphocytes in vitro. Additionally, a growing body of work has identified MIP-3 alpha as a functional ligand of GPR-CY4 orphan receptor or CCR6 and given useful information about in vivo function.
Biochem/physiol Actions
Expression of the chemokine is detected in the lymphoid tissues, fetal lung and fetal liver. Recombinant and native MIP-3α are known chemoattractants of lymphocytes in vitro. Additionally, a growing body of work has identified MIP-3α as a functional ligand of GPR-CY4 orphan receptor or CCR6 and given useful information about in vivo function.
Analysis Note
The biological activity is measured by its ability to induce calcium flux in HEK293 cells stably expressing CCR-6 and for chemotaxis of mouse BaF/3 cells transfected with hCCR6.
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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