M1194

Anti-MTA2/MTA1L1 (RT-16) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Synonym(s): Anti-Metastasis-associated Gene
• MDL number: MFCD07370406
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: IgG fraction of antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen 73 kDa
• species reactivity: human, rat, mouse
• technique(s): immunoprecipitation (IP): 1-2 μL using 293-T cell lysate; indirect immunofluorescence: 1:100-1:200 using methanol-acetone fixed C2 myoblasts; western blot: 1:2,000-1:4,000 using nuclear extracts of MCF7 cells
• UniProt accession no.: O94776
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... MTA2(9219) ; mouse ... Mta2(23942) ; rat ... Mta2(361724)

Description

General description

Metastasis-associated protein (MTA2) comprises bromo-adjacent homology (BAH), Egl-27 and MTA1 homology 2 (ELM2), Swi3, Ada2, N-Cor, and TFIIIB (SANT), and an atypical GATA zinc finger domain. It is a phosphorylated protein. MTA2 gene is mapped to human chromosome 11q12.3. Human MTA2 shares approximately 80% overall homology to human MTA1 and MTA3 proteins, with the C-terminus being more divergent than the N-terminus.

Specificity

Anti MTA2/MTA1L1 (RT-16) specifically recognizes MTA2/MTA1L1.

Immunogen

synthetic peptide corresponding to amino acids 626-641 of human MTA2/MTA1L1, conjugated to KLH via an N-terminal added cysteine residue. The immunizing peptide is conserved in human, mouse, and rat, and is not present in other members of the family.

Application

Anti-MTA2/MTA1L1 (RT-16) antibody produced in rabbit has been used in:

• tandem immunoprecipitation[1]
• immunoblotting
• immunofluorescence

Biochem/physiol Actions

Metastasis-associated protein (MTA2) mediates cytoskeletal reorganization. It interacts with the histone deacetylases (HDAC) within the nuclear remodeling and deacetylation complexes namely, nucleosome remodeling and deacetylase (Mi2/NuRD). This interaction mediates transcriptional repression. Also called p53 target protein in deacetylase complex (PID), it regulates p53 function and represses p53-dependent transcriptional activation, growth arrest and apoptosis. MTA2 also favors tumor growth and migration.

Physical form

Solution in 0.01 M phophate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)