M1074
MYLK3, active, GST tagged human
PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym(s):
MGC126319, MGC126320, MLCK2, caMLCK
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About This Item
UNSPSC Code:
12352204
NACRES:
NA.32
recombinant
expressed in baculovirus infected Sf9 cells
Quality Level
product line
PRECISIO® Kinase
assay
≥70% (SDS-PAGE)
form
buffered aqueous glycerol solution
specific activity
7-11 nmol/min·mg
mol wt
~135 kDa
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... MYLK3(91807)
General description
Biochem/physiol Actions
MYLK3 (or myosin light chain kinase 3) plays a key role in rate of cross-bridge recruitment in cardiac myocytes. MYLK3 is upregulated in failing human myocardia and expression of MYLK3 correlates with the pulmonary arterial pressure of patients with heart failure. Knockdown of MYLK3 with small interfering RNAs in cultured rat cardiomyocytes decreases Myl2 phosphorylation and impairs epinephrine-induced activation of sarcomere reassembly. Recombinant murine MYLK3 shows specificity for Myl2 (Mlc2v) and the phosphorylation is calcium- and calmodulin-dependent.
MYLK3 (myosin light chain kinase 3) plays a major role in the modulation of actin cytoskeleton and immune response signalling.[3] It controls the epithelial tight junctions and the structure of actomyosin belt.[2] MYLK3 also participates in the intestinal TJ (tight junction) permeability.[4]
MYLK3 (myosin light chain kinase 3) plays a major role in the modulation of actin cytoskeleton and immune response signalling.[3] It controls the epithelial tight junctions and the structure of actomyosin belt.[2] MYLK3 also participates in the intestinal TJ (tight junction) permeability.[4]
Physical form
Supplied in 50 mM Tris-HCl, pH 7.5, with 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, and 25% glycerol.
Legal Information
PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Identification of MYLK3 mutations in familial dilated cardiomyopathy.
Tobita T, et al.
Scientific Reports, 7(1), 17495-17495 (2017)
Methylation of MYLK3 gene promoter region: a biomarker to stratify surgical care in ovarian cancer in a multicentre study.
Phelps D L, et al.
British Journal of Cancer, 116(10), 1287-1287 (2017)
Cyclic stretch disrupts apical junctional complexes in Caco-2 cell monolayers by a JNK-2-, c-Src-, and MLCK-dependent mechanism.
Samak G, et al.
American Journal of Physiology: Gastrointestinal and Liver Physiology, 306(11), G947-G958 (2014)
Identification of cardiac-specific myosin light chain kinase.
Chan J Y, et al.
Circulation Research, 102(5), 571-580 (2008)
Osamu Seguchi et al.
The Journal of clinical investigation, 117(10), 2812-2824 (2007-09-22)
Marked sarcomere disorganization is a well-documented characteristic of cardiomyocytes in the failing human myocardium. Myosin regulatory light chain 2, ventricular/cardiac muscle isoform (MLC2v), which is involved in the development of human cardiomyopathy, is an important structural protein that affects physiologic
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