immunohistochemistry (formalin-fixed, paraffin-embedded sections): 10-20 μg/mL using heat retrieved, human prostate sections western blot: 1-2 μg/mL using human A431 cell extract
Maspin gene is located on chromosome 18q21.3 in a cluster of genes encoding members of the ovalbumin family of serine protease inhibitors. Expression of maspin was found also in human cornea in the epithelium, endothelium and stroma cells.
Immunogen
synthetic peptide corresponding to amino acids 333-348 of human maspin with a C-terminal added cysteine, conjugated to KLH.
Application
Anti-Maspin antibody produced in rabbit has been used in immunoblotting and immunohistochemistry.
Biochem/physiol Actions
Maspin is a serine protease inhibitor (serpin) with tumor suppressive activities. Maspin protein was identified in a search for tumor suppressor genes that are defective in human breast carcinoma cells. Maspin is expressed in normal mammary epithelial cell strains, but not in most mammary tumor cell lines. Re-expression of maspin in mammary carcinoma cells does not alter the growth properties of the cells in vitro, but reduces their ability to induce tumors, to metastasize in nude mice, and to invade a basement membrane matrix in vitro. The molecular mechanism of maspin action is not clear. Using a two-hybrid system, it has been found that heat shock protein 90, glutathione S-transferase (GST), and heat shock protein 70 interacted with maspin with the highest frequencies. This protein exerts its tumor suppressive role, at least in part, by blocking the activity of pericellular uPA (urokinase Plasminogen Activator).
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide and 1% inactivated bovine serum albumin.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
