M0819

Anti-MTA3L antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Synonym(s): Anti-Metasisis-associated Gene
• MDL number: MFCD07370407
• UNSPSC Code: 12352203

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: IgG fraction of antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen 75 kDa
• species reactivity: human
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• technique(s): microarray: suitable; western blot: 1:1,000-1:2,000 using transfected cell extracts of HEK 293-T cells
• UniProt accession no.: Q9BTC8
• shipped in: dry ice
• Storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... MTA3(57504)

Description

General description

Metastasis associated 1 family member 3 (MTA3) is a component of the Mi-2/ nucleosome remodeling and deacetylase (NURD) complex. It comprises the bromo-adjacent homology (BAH) GATA-zinc finger (ZF), Egl27/MTA1 (ELM), SW13, ADA2, N-CoR and TFIIIB domain (SANT) domains. The MTA3 gene is located on human chromosome 2p21 and encodes for two isoforms (MTA3 and MTA3L).

Specificity

Anti-MTA3L recognizes specifically human MTA3L.

Immunogen

synthetic peptide corresponding to amino acids 551-568 of human MTA3L, conjugated to KLH via an N-terminal added lysine residue. The immunizing sequence is not present in other members of the family, MTA1 and MTA2.

Application

Anti-MTA3L antibody produced in rabbit may be used in immunoblotting.

Biochem/physiol Actions

Metastasis associated 1 family member 3 (MTA3) is an estrogen receptor (ER)-regulated component of the Mi-2/ nucleosome remodeling and deacetylase (NURD) complex transcriptional corepressor complex. This complex is crucial for the initiation of hematopoiesis. Post estrogen binding to the estrogen receptor, MTA3, in conjunction with other NuRD components, decreases expression of the transcriptional repressor Snail, ultimately modulating E-cadherin expression and maintenance of normal epithelial architecture. Loss of MTA3 or ER leads to deregulated expression of Snail, transcriptional repression of E-cadherin, and a consequent predisposition to invasive cell growth in breast epithelial cancers. MTA3 plays a role in B cell fate determination, through interaction with the transcription factor B cell lymphoma 6 (BCL-6). It also regulates epithelial-mesenchymal transitions.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in frost-free freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable