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M0404

Sigma-Aldrich

Murashige and Skoog Basal Medium

powder, suitable for plant cell culture, with Gamborg′s vitamins

Synonym(s):

MS0 Medium, MSO Medium, Murashige and Skoog (Gamborg Modified) Basal Media, MS Basal Medium

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1 L
$15.10
10 L
$44.20

About This Item

MDL number:
UNSPSC Code:
12352207
NACRES:
NA.72

$15.10


Available to ship onMay 05, 2025Details


Request a Bulk Order
Notify Me

Get notified when this item is ready to ship via email.

Quality Level

form

powder

technique(s)

cell culture | plant: suitable

application(s)

agriculture

shipped in

ambient

storage temp.

2-8°C

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Application

Murashige and Skoog Basal Medium has been used:
  • to germinate the seeds for seedling growth and germination studies[1]
  • in the suspension culture of Nicotiana plumbaginifolia for transformation[2]
  • in the root culture of hairy clone A. artemisiifolia T4[3]

Formula variant

With the macro- and micronutrients as described by Murashige and Skoog (1962) and the vitamins as described by Gamborg, et al. (1968).

Media Formulation

Quantity

Formulated to contain 4.4 grams of powder per liter of medium.

Preparation Note

Murashige and Skoog medium can be reconstituted from powder or by combining products that are major components of complete M&S medium, such as macronutrient, micronutrient and vitamin mixtures.
Murashige and Skoog medium (M0404) contains the macro- and micronutrients of M&S basal medium as described by Gamborg, et al (1968). It can be supplemented with organics and with auxins (IAA) and cytokinins (Kinetin) to generate a complete medium for growth plant tissue culture.

pictograms

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Warning

hcodes

Hazard Classifications

Eye Irrit. 2 - Ox. Sol. 3

Storage Class

5.1B - Oxidizing hazardous materials

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Identification of a Golgi-localised GRIP domain protein from Arabidopsis thaliana
Gilson PR, et al.
Planta, 219(6), 1050-1056 (2004)
Crescencio Bazaldúa et al.
PloS one, 14(9), e0222464-e0222464 (2019-09-13)
Ten Hyptis suaveolens hairy root lines were established by infecting nodal explants with K599+pGus-GFP+ and ATCC15834+pTDT strains from Agrobacterium rhizogenes. Genetic transformation was confirmed by epifluorescence and plagiotropic hairy root growth in absence of growth regulators. Cytotoxicity was determined using
Statistical analysis of elicitation strategies for thiarubrine A production in hairy root cultures of Ambrosia artemisiifolia
Bhagwath SG and Hjortso MA
Journal of Biotechnology, 80(2), 159-167 (2000)
John E McLaughlin et al.
PloS one, 10(6), e0130204-e0130204 (2015-06-10)
Fusarium head blight (FHB) or scab is one of the most important plant diseases worldwide, affecting wheat, barley and other small grains. Trichothecene mycotoxins such as deoxynivalenol (DON) accumulate in the grain, presenting a food safety risk and health hazard
Ken-Der Wang et al.
The Plant cell, 32(1), 166-185 (2019-11-07)
Multiple long-distance signals have been identified for pathogen-induced systemic acquired resistance, but mobile signals for symbiont-induced systemic resistance (ISR) are less well understood. We used ISR-positive and -negative mutants of maize (Zea mays) and the beneficial fungus Trichoderma virens and

Questions

1–2 of 2 Questions  
  1. What is electric conductivity of Murashige-Skoog culture solution?

    1 answer
    1. The Murashige and Skoog media products have not been tested for conductivity.

      Helpful?

  2. I would like to know if there is any problem in irradiating with UV light, media culture prepared with the medium.

    1 answer
    1. Testing protocols for plant culture media include sterilization by autoclaving. Heat-labile components are filter-sterilized and added after autoclaving, prior to asceptic dispensing to sterile vessels. UV sterilization has not been investigated as a sterilizing technique for culture media products, although this does appear to be growing in popularity. Keep in mind that UV would not melt agar used in solid media formats. Autoclaving or boiling would still be required. Please see the link below for a publication that may offer additional information:
      https://pubmed.ncbi.nlm.nih.gov/25044686/#:~:text=Ultraviolet%20(UV)%20irradiation%20is%20a,sterilization%20of%20cell%20culture%20media.

      Helpful?

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