To reconstitute, it is recommended to dissolve the contents of one vial in 10 mL of deionized, purified water. After reconstitution, filter-sterilize through a 0.2 µm low-protein binding membrane.
L6170
β-Lactamase
recombinant, expressed in E. coli
Synonym(s):
Carbapenemase, Cephalosprinase
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recombinant
expressed in E. coli
Quality Level
form
powder
specific activity
≥20 U/mg (with cephalosporin C)
≥400 U/mg (with benzylpenicilin)
storage temp.
2-8°C
General description
β-Lactamase produced by bacteria is closely related to the penicillin binding proteins. β-Lactamase hydrolyzes β-lactum antibiotics and is the prime cause of resistance development by bacteria.[1] There are four subclasses of β-Lactamases. Classes A, C and D form an acyl-enzyme via active site serine residue. Class B β-lactamases are metalloenzymes with zinc ion at their active site for β-lactam hydrolysis.[2] Mutations in the β-lactamases has resulted in the generation of extended-spectrum β-lactamases (ESBLs).[3] As close to 900 types of β-Lactamases are produced by microbes.[4]
Application
β--lactamase is used to inactivate β-lactam antibiotics by breaking open the β-lactam ring. β--lactamase is used to study antibiotic resistance and resistance suppression[5]. Product L6170 is recombinantly produced based on the sequence of the enzyme from Pseudomonas aeruginosa, and is expressed in E. coli.
Biochem/physiol Actions
β--lactamase inactivates β-lactam antibiotics by breaking open the β-lactam ring[5]. Typical analysis for other substrates: ceftriaxon 2-4 un/mg; cefazolin 2-4 un/mg; ceftazidime 1.5-3 un/mg; cefoxitin 0.35-0.7 un/mg; cefepime 1.2-2.4 un/mg; cefuroxime 1.5-3 un/mg; cefotaxime 1-2 un/mg, oxacillin 4-8 un/mg; imipenem 1.2-2.4 un/mg; and meropenem 3-6 un/mg
Typical analysis for other substrates: ceftriaxon 2-4 un/mg; cefazolin 2-4 un/mg; ceftazidime 1.5-3 un/mg; cefoxitin 0.35-0.7 un/mg; cefepime 1.2-2.4 un/mg; cefuroxime 1.5-3 un/mg; cefotaxime 1-2 un/mg, oxacillin 4-8 un/mg; imipenem 1.2-2.4 un/mg; and meropenem 3-6 un/mg
Unit Definition
One unit will hydrolyze 1.0 μmole substrate per min (βI: benzylpenicillin; βII: cephalosporin C) in 50 mM phosphate buffer, pH 7.0 containing 10 μM zinc chloride at 25 ºC.
Physical form
Lyophilized powder containing sodium chloride and potassium phosphate.
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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